Prestained Protein Marker Ⅸ (2-40 KDa)

Western Protein Marker Ⅸ is a prestained mixture of seven high-purity recombinant proteins and peptides, The bands display in Tris-Glycine gel range from 2-40 kDa (~2,~4.5,~8,~13,~20,~30,~40 kDa), of which 30 kDa and 8 kDa are rose-red bands, others are blue bands. which is convenient for monitoring of electrophoresis and transfer efficiency. It is suitable as a protein molecular weight standard for SDS-PAGE and Western Blot.

Ship with wet ice; Store at -20℃, valid for 12 months.

1. This product is ready to use with no heating, diluting or additional reducing agent necessary. Thaw at room temperature and mix gently and thoroughly.

2. Take 5-10 μL (G2090) and samples for protein electrophoresis; It is suggested that qualified laboratories can determine the appropriate sample volume by their own experimental conditions and habits when using this product for the first time, which can not only save costs, but also obtain better experimental figures. Small molecular weight proteins electrophoresis using Tris-Tricine gel system (recommended G2159), other gel systems can not separate proteins below 10 kDa; WB transfer membrane need to use 0.22 μM pore size PVDF membranes (reference conditions: 300 mA, 30 min).

3. Prestained Protein Marker Ⅸ should be stored at -20℃ immediately after use (5-10 μL recommended for storage and use).

1. Do not boil the protein marker, which can lead to degradation or decolourisation of protein bands.

2. Small molecular weight prestained Marker is recommended to use Tris-Tricine gel system, 100 V electrophoresis for 4-5 h, need to be ice bath throughout the whole process; transfer membrane need to use 0.22 μM pore size PVDF membrane, the reference conditions: 300 mA, 30 min.

3. This prestained protein marker can be stored at room temperature for ≥20 days; store at 2-8℃ for ≥20 months; store at -20℃ for ≥12 months.

4. The molecular weight of the reference prestained protein in the figure is calibrated from the molecular weight of the non-prestained protein.

5. For your safety and health, please wear safety glasses, gloves, or protective clothing.

Pic. 1. Band profile of Prestained Protein Marker Ⅸ

For Research Use Only!