Product Information
|
Product Name |
Cat. No. |
Spec. |
|
5×Tris-Glycine SDS-PAGE Running Buffer |
G2162-1L |
1 L |
Product Description/Introduction
This product is a 5x concentrated Tris-Glycine SDS-PAGE protein gel electrophoresis buffer. The main components are 125 mM Tris, 1.25 M glycine, and 0.5% SDS. It needs to be diluted 5 times before use.Take 200 mL of this product and mix thoroughly with 800 mL of deionized water or distilled water to obtain a working solution with the main components of 25 mM Tris, 250 mM glycine, 0.1% SDS, pH 8.0-8.6 at 25°C. It is suitable for Tris-Glycine system protein gel electrophoresis.
Storage and Shipping Conditions
Ship and store at room temperature; Valid for 12 months.
Assay Protocol/Procedures
Take 200 mL of this product and mix thoroughly with 800 mL of deionized water or distilled water to obtain a 1000 mL 1x working solution with 25 mM Tris, 250 mM glycine, 0.1% SDS, pH 8.0-8.6 at 25°C.
Note
1. Please use the solution as soon as possible after preparation.
2. This electrophoresis buffer is only suitable for Tris-Glycine system protein gel electrophoresis and can be used with our company's products G2003, G2037, G2041, G2042, G2043, G2044, G2045, G2060, G2061, G2062, G2063, G2064.
3. Crystallization may occur at lower environmental temperatures, which is a normal phenomenon. It can be heated at 37°C and shaken several times until completely dissolved before dilution and use.
4. For better electrophoresis results, reuse is not recommended.
For Research Use Only!
