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Immunoprecipitation Kit (Protein G Magnetic Beads)Cat.No.:G2210-50Tread more
Brand:Servicebio
Spec.: 50 T -
SweMagrose Protein A Antibody Purified Magnetic BeadsCat.No.:G3656-5MLread more
Brand:Servicebio
Spec.: 5 mL (Protein A Antibody Purified Magnetic Beads) -
Coomassie (Bradford) Protein Quantitative Assay KitCat.No.:G2001-250MLread more
Brand:Servicebio
Spec.: 250 mL -
RIPA Lysis Buffer (Strong)Samples of animals, plants, cells, fungi or bacteria are applicable; Can be used for PAGE, WB, IP, COIP and other experimentsread more
Cat.No.:G2002-30ML
Brand:Servicebio... -
50×CocktailEffectively protect the integrity of proteins and ensure experimental results.read more
Cat.No.:G2006-250UL
Brand:Servicebio
Spec.: 30 mL (Strong) -
Phosphoprotease InhibitorEspecially, it can effectively protect the integrity of phosphorylated protein and guarantee the experimental resultsread more
Cat.No.:G2007-1ML
Brand:Servicebio
Spec.: 30 mL... -
PMSF (100mM)Effectively protect the integrity of proteins and ensure experimental results.read more
Cat.No.:G2008-1ML
Brand:Servicebio
Spec.: 30 mL (Strong) -
Prestained Protein Marker II (10-200 KDa)Cat.No.:G2058-250ULread more
Brand:Servicebio
Spec.: 250 μL (12-154 kDa) (WB Experimental Exposure)
Use this buffer with mechanical cell lysis such as homogenization with a Dounce homogenizer. Store up to 6 months at 4°C. Immediately before use add protease inhibitors. Epitopes of...
A good strategy is first to lyse cells using a relatively strong buffer such as radioimmunoprecipitation assay buffer (commonly referred to as RIPA buffer) containing the...
Remove the supernatant and add 400 μL of buffer made with protease inhibitors (can be the same as the lysis buffer). The slurry is now ready for use. It can be stored at 4°C for a few days; for...
IP lysis Buffer 12.5 ml 1M NaCl (250mM) 2.5 ml 1M Tris (50mM) 500ul 0.5M EDTA (5mM) 2.5ml 10% NP-40 32 ml dH20 Protein A Beads Resuspend 400 mg of Protein A beads in 10 ml of distilled H2O. Mix well...
Pierce IP Lysis Buffer is a ready-made formula for extracting proteins from mammalian cells for immunoprecipitation and pull-down assays. It is compatible with many protein assays and does not...
You should develop an appropriate lysis strategy to maximize structural integrity. The ionic strength (salt concentration), choice of detergent, and pH of the lysis buffer may affect the...
