50×Cocktail

Many of the cellular proteins are kept separate from proteolytic enzymes. Disruption of cellular and tissue architecture during protein extraction distorts the in vivo state by making all proteins potentially accessible for degradation or modification by endogenous proteases. That resulting unregulated proteolytic activity can reduce protein yield and function. Protease inhibitors can be added to the lysis reagents in order to prevent degradation of extracted proteins, and to obtain the best possible protein yield and activity following cell lysis.

50×Cocktail Protease Inhibitor (EDTA-free) is a concentrated stock solution containing several different proteases inhibitor compounds (AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, Pepstatin A) that can be added to samples to prevent protein degradation during cell lysis and protein extraction.

Ship with dry ice. Store at -20°C, valid for 12 months.

This product is 50× concentrated solution. 20 µL ofconcentrated solution is added to 1 mL ofprotein lysis solution to ensure complete protection for protein extracts. It must be added before useand cannot be stored to prevent invalidation. At the same time, we provide protein lysis reagents (G2002, G2033).

For your safety and health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!