Product Introduction
|
Product Name |
Cat. No. |
Spec. |
|
Phosphoprotease Inhibitor |
G2007-1ML |
1 mL |
Product Description/Introduction
This product is an upgraded version of a broad-spectrum phosphorylated protease inhibitor. Compared to the previous version, it has been enhanced with additional components, including Sodium fluoride, Sodium orthovanadate, Sodium pyrophosphate, Imidazole, Sodium molybdate, Sodium tartrate, and β-Glycerophosphate, all dissolved in water. It effectively inhibits common intracellular phosphatases such as serine phosphatases, tyrosine phosphatases, threonine phosphatases, as well as acid phosphatases and alkaline phosphatases, offering superior performance.
Furthermore, the product has been optimized from two separate reagents into a single reagent tube, making it more convenient to use.
This product is suitable for the extraction of proteins from various biological samples and is compatible with subsequent protein quantification, immunoblotting experiments (such as WB, Co-IP, pull-down), and more. It comes as a 100× concentrate, meaning 1 mL of the phosphorylated protease inhibitor can be used to prepare 100 mL of lysis buffer.
Storage and Shipping Conditions
Ship with dry ice;Store at -20℃, valid for 12 months.
Product Components
|
Component Number |
Component |
G2007-1ML |
|
G2007 |
Phosphoprotease Inhibitor |
1 mL |
|
Manual |
||
Assay Protocol/Procedures
1.Before use, thaw and keep this product at room temperature.
2.Use it at a 1:100 ratio (volume ratio), which means adding 10 μL of phosphorylated protease inhibitor to every 1 mL of lysis buffer.
3.Add it immediately before use. Do not pre-mix it with the lysis buffer and store it, as this may cause it to lose effectiveness. It is recommended to use it in conjunction with our lysis buffers (G2002, G2033 recommended).
Precautions
1.This product is a 100× concentrate. After being stored at low temperatures and thawed, trace crystalline precipitates may appear, which is normal. After fully dissolving and mixing, it can be used normally.
2.The phosphatase content varies in different samples. The 1:100 ratio is the recommended standard usage. If the phosphatase content in the sample is high, you may appropriately increase the concentration of the inhibitor.
3.When using it in combination with protease inhibitors (such as G2006), please avoid using EDTA. EDTA can affect the inhibitory effect of the phosphorylated protease inhibitor.
4.For your safety and health, please wear a lab coat and disposable gloves while operating.
For Research Use Only!
