Acid-fast Staining Solution

This product is suitable for staining acid-fast bacteria and can be used to distinguish acid-fast bacteria from non-acid-fast bacteria. The basic principle is that there is a layer of lipids surrounding peptidoglycan on the surface of acid-resistant bacteria such as tuberculosis bacteria and Mycobacterium leprae, so it is not easy to stain when stained, and once the stain is promoted by some methods, it is difficult to be decolorized by acid decolorizing agent, hence the name acid-fast staining.

This set of acid-fast staining solution is suitable for detecting acid-fast bacteria in tissues. After staining, the nuclei were blue and acid-fast bacteria were purplish red.

Store and transport at room temperature, valid for 12 months.

Prepare hematoxylin differentiation solution (G1039), hematoxylin bluing solution (G1040), xylene, ethanol, neutral gum, etc.

1. Paraffin sections are dewaxed to water.

2. Sections into acid-fast staining solution A, cover and soak for 30 min, and rinse with running water for 2 min.

3. The sections enter the acid-fast staining solution B for 1-2 seconds, and then it is washed with water. Repeat the steps of differentiation and washing, and observe with microscope in time. The acid-fast bacteria can differentiate into purple red, and the tissue background is basically colorless.

4. The slices were stained with acid-fast staining solution C for 30 seconds, then differentiated with hematoxylin differentiation solution for 2 seconds, washed with tap water, and then blued with hematoxylin bluing solution for 3-5 seconds, washed with tap water.

5. The sections were dehydrated by 3 cylinders of absolute ethanol for 5 min each, then transparent by xylene for 5 min, and sealed with neutral gum.

1. The staining time of acid-fast staining solution A can be appropriately prolonged when the room temperature is low in winter.

2. Tighten the cap in time after using the reagent to prevent volatilization of active ingredients or product deterioration.

3. Each set of staining solution can be used for staining (dip staining) approximately 80 sections. Please replace the stain with a new one when the tissue or cell coloring is significantly lighter or abnormal.

4. Wear a lab coat and disposable gloves during operation.

Acid-fast bacteria are purplish red with blue nuclei.

For Research Use Only!