Feulgen Stain Kit

The Feulgen reaction is the traditional way to visualize DNA. The basic principle is that the aldehydes formed by the calculation of DNA combine with Schiff reagent to form a magenta derivative. Feulgen staining is based on this principle. It is specific for DNA, and the nuclei of bacteria or cells can be observed under ordinary light microscopy after staining with this method.

This product Feulgen dyeing solution set contains 4 components. The active component of Feulgen dye solution A is hydrochloric acid, Feulgen dye solution B is colorless fuchsin dye solution, Feulgen dye solution C is sodium sulphite, Feulgen dye solution D is fast green, the concentration is 0.16%. After staining, the nuclear DNA was purplish red, and the cytoplasm and other components were light green

Wet ice transportation; Among them, Feulgen dye solution B needs to be stored at 4℃ away from light, and the rest can be stored at room temperature. It is valid for 12 months.

1. The paraffin sections are dewaxed to water.

2. After the sections were moistened in Feulgen dye solution A at room temperature, the sections were treated with Feulgen dye solution A at 60℃ for 10-30 min, and then the sections were treated with Feulgen dye solution A at room temperature for 3-5 s. The sections were rinsed 3 times with pure water for 5-10 s each time.

3. After the sections were covered in Feulgen dye solution B and stained for 60-90 min, the sections were removed and directly immersed in Feulgen dye solution C twice, 2 min each time. Then the sections were removed and rinsed with running water for 5 min.

4. Sections were counterstained with Feulgen dye solution D for 5-30 s, and then dehydrated by 3 cylinders of absolute ethanol for 5 s, 5 s, and 30 s, respectively. The dyeing time was adjusted according to the degree of staining.

5. The slices were transparent through xylene for 5 min and sealed with neutral gum.

Note: Prepare absolute ethanol, xylene, neutral gum, etc.