Modified Giemsa Staining Kit

 

Product Name	Cat.No.	Spec..
Modified Giemsa Staining Kit	G1079-100T	100T

 

 

The modified Giemsa staining solution is composed of azurin, eosin and fluorescent pink B. Azurin is an alkaline dye that can bind to basophilic particles in cells such as nuclear proteins or lymphocyte cytoplasm to be dyed blue-purple; eosin and fluorescent pink B are acidic dyes, which can bind to eosinophilic particles in cells such as cytoplasm and be dyed pink, while neutral particles can bind to eosin, fluorescent pink B and azurin in an equal point state, showing lavender.

This product is modified Giemsa staining solution, adding fluorescent pink B, which can better distinguish the acidic particles in eosinophils. Meanwhile, the staining differentiation solution can also make the staining result more contrast, and can present a clear staining image of cells under a light microscope. It is mainly suitable for cell smear and paraffin/frozen tissue section with good staining effect and clear staining.

 

 

Store at room temperature away from light for 24 months.

 

 

Component Number	Component	G1079-100T
G1079-1	Modified Giemsa staining stock solution	25 mL
G1079-2	Modified Giemsa staining Diluent	250 mL
G1079-3	Modified Giemsa staining differentiation solution	250 mL
Manual		1 pc

 

 

1. Cell smears (blood, bone marrow and alveolar lavage fluid, etc.)：

1) Smears were taken, dried naturally, and fixed (blood smears were air-dried and fixed by soaking in methanol for 15 min; alveolar lavage fluid and bone marrow smears were air-dried and fixed by soaking in acetone for 1 min);

2) Take 1 part of modified Giemsa staining stock solution and add 9 parts of the modified Giemsa diluent to mix thoroughly, which is the working liquid;

3) Add the working liquid, stain at room temperature for 10 min, and use 100-500 μL for each section;

4) Rinse slowly from one end of the slide with distilled water to remove the stain on the surface of the slide;

5) Put in oven for drying after microscope inspection;

6) Slice into clean xylene transparent for 1min and seal with neutral gum.

2. Paraffin/Frozen Tissue Section:

1) Pretreatment

a) Paraffin section dewaxing to water: put the slides into environment-friendly dewaxing solution I and II for 15 minutes each; Anhydrous ethanol I, II and III for 5 minutes each; 75% ethanol for 2 minutes; Water washing for 1 minute;

b) Frozen slices stored at -20℃, removed and restored to room temperature tissue to dry, fixed with methanol for 1min; Freshly cut frozen sections should be dyed after OCT is completely dried to prevent peeling;

2) Take 1 part of modified Giemsa staining stock solution and add 9 parts of the modified Giemsa diluent to mix thoroughly, which is the working liquid;

3) Add the working liquid, stain at room temperature for 10 min; For frozen section, the dyeing time and dilution ratio of stock solution can be adjusted according to the depth of dyeing. The dosage of each section is 100 μL;

4) Rinse slowly from one end of the slide with distilled water to remove the stain on the surface of the slide;

5) Shake off the water and put it into the modified Giemsa staining differentiation solution for 2-3 times;

6) Shake off the water and put it into the pure water 2-3 times;

7) Put in oven for drying after microscope inspection;

8) Slice into clean xylene transparent for 1 min and seal with neutral gum.

 

 

Cell Type	Cytoplasmic Color
Mature Red Blood Cell	Pink
Neutrophil	Lavender
Eosinophil	red
Basophil	Blue-Purple
Lymphocyte, Monocyte	Blue-Purple

 

 

1. Blood, bone marrow and alveolar lavage fluid smears should be evenly spread, and fixed after natural drying, otherwise the cells are easy to fall off during the staining process.

2. Modified Giemsa staining stock solution is toxic to human body and flammable. Please be careful when operating, and pay attention to effective protection to avoid contact with human body or inhalation of human body.

3. If the dyeing is too light, it can be redyed, and the operation of redyed is the same as that in the manual. If dyed too deep can be soaked in pure water.

4. The optimal dilution ratio of Modified Giemsa staining stock solution can be adjusted according to different tissues or smears.

5. pH value has an impact on cell staining, dyeing slides must be cleaned to avoid acid and alkali pollution affecting the staining results.

6. The working liquid should be prepared and used immediately, otherwise precipitation will occur and affect the dyeing result.

7. Each set of staining solution can be used to stain approximately 100 sections (drop staining). Replace the stain with a new one when the tissue or cell coloring is significantly lighter or abnormal.

 

For Research Use Only!