Product Information
|
Product Name |
Cat. No. |
Spec. |
|
Ham's F-12, GlutaPlus |
G4562-500ML |
500 mL |
Product Description/Introduction
Ham's F-12 (Ham's F-12 Nutrient Mixture) is based on Ham's F-10 nutrient mix and was originally designed for serum-free culture of CHO cells. Ham's F-12 is often used as a basal culture medium for serum-free cultures and is particularly suitable for single cell cultures and clonogenic cultures at low serum levels. It is also widely used in the culture of tumor cells and primary cells by adding serum, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myogenic cells, chicken embryonic cells, etc. In addition, Ham's F-12 is mixed with DMEM in equal volume to obtain a more nutrient-rich DMEM/F-12 medium.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors.Need to add serum or serum-free additives depending on the cell type.
This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 10 mM D-Glucose, 1.0 mML-Alanyl-L-Glutamine, 1.0 mM sodium pyruvate and phenol red indicator, without HEPES.
Storage and Shipping Conditions
Ship at room temperature;Store away from light at 2-8℃, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, glovesand protective clothing.
For Research Use Only!
G4562 Ham's F-12 Formula
|
Components |
Concentration |
Components |
Concentration |
||
|
mg/L |
mM |
mg/L |
mM |
||
|
Amino acids |
Inorganic salts |
||||
|
Glycine |
7.507 |
0.1 |
Potassium Chloride (KCl) |
223.6 |
2.98 |
|
L-Alanine |
8.90 |
0.1 |
Sodium Bicarbonate (NaHCO3) |
1176 |
14 |
|
L-Arginine hydrochloride |
210.66 |
1.0 |
Sodium Chloride (NaCl) |
7599 |
131.02 |
|
L-Asparagine-H2O |
15.02 |
0.1 |
Dibasic sodium phosphate(Na2HPO4) (anhyd.) |
141.96 |
1.0 |
|
L-Aspartic Acid |
13.31 |
0.1 |
CalciumChloride (CaCl2)(anhyd.) |
33.22 |
0.30 |
|
L-Cysteine hydrochloride-H2O |
35.05 |
0.20 |
Copper Sulfate Pentahydrate (CuSO4·5H2O) |
0.0025 |
0.00001 |
|
L-GlutamicAcid |
14.71 |
0.1 |
Iron Sulfate Heptahydrate (FeSO4·5H2O) |
0.834 |
0.003 |
|
L-Alanyl-L-Glutamine |
217.2 |
1.0 |
Magnesium Chloride (MgCl2)(anhyd.) |
57.35 |
0.60 |
|
L-Histidine hydrochloride-H2O |
20.96 |
0.1 |
Zinc Sulfate Heptahydrate (ZnSO4·7H2O) |
0.862 |
0.003 |
|
L-Isoleucine |
4.005 |
0.031 |
Other components |
||
|
L-Leucine |
13.12 |
0.1 |
D-Glucose (Dextrose) |
1802.0 |
10.01 |
|
L-Lysine hydrochloride |
36.5 |
0.199 |
Phenol red |
1.13 |
0.00319 |
|
L-Methionine |
4.5 |
0.03 |
Sodium pyruvate |
110 |
1.0 |
|
L-Phenylalanine |
5.0 |
0.03 |
Hypoxanthine |
4.083 |
0.03 |
|
L-Proline |
34.5 |
0.3 |
Linoleic Acid |
0.084 |
0.0003 |
|
L-Serine |
10.5 |
0.1 |
Alphalipoic Acid |
0.210 |
0.00102 |
|
L-Threonine |
11.9 |
0.10 |
Diaminobutane dihydrochloride |
0.161 |
0.001 |
|
L-Tryptophan |
2.0 |
0.01 |
Thymidine |
0.701 |
0.00289 |
|
L-Tyrosine Disodium Salt |
7.8 |
0.0298 |
|||
|
L-Valine |
11.7 |
0.1 |
|||
|
Vitamins |
|||||
|
Biotin |
0.0073 |
0.0000299 |
|||
|
Choline chloride |
14 |
0.1 |
|||
|
D-Calcium pantothenate |
0.50
|
0.001048 |
|||
|
Folic Acid |
1.30 |
0.002948 |
|||
|
Niacinamide |
0.04 |
0.000295 |
|||
|
Pyridoxine hydrochloride |
0.06 |
0.000291 |
|||
|
Riboflavin |
0.04 |
0.000098 |
|||
|
Thiamine hydrochloride |
0.30 |
0.000890 |
|||
|
Vitamin B12 |
1.40 |
0.001033 |
|||
|
i-Inositol |
18 |
0.1 |
|||
