Product Introduction
Servicebio'sTransfer Sponge (Cat. No. G6002 Series) is a premium support material meticulously engineered for Western blot protein transfer applications. Designed to streamline the electrotransfer process, these sponges ensure consistent, high-quality results across diverse protein sizes, particularly enhancing the transfer of large molecules (>150 kDa). Compatible with both semi-dry and wet transfer systems, our Transfer Sponge is trusted by leading research institutions and pharmaceutical laboratories worldwide.
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Product Name |
Cat.No. |
Spec. |
|
Transfer Sponge |
G6002 |
2 pcs(7×9 cm) |
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G6002-15 |
15 pcs (7×9 cm) |
|
|
G6002-20 |
20 pcs(7×9 cm) |
Key Attributes & Specifications
Dimensions: 7×9 cm (pre-compressed thickness: 5.0±0.2 mm; expands to 6–7 mm upon buffer immersion)
Certifications: Manufactured under ISO 9001, ensuring batch-to-batch reliability.
Core Advantages
Anti-Short Circuit Design: Laser-cut edges prevent membrane curling and current leakage, reducing transfer failures.
Enhanced Buffer Flow: Proprietary porous architecture accelerates ion movement, boosting transfer efficiency for low-abundance proteins.
Precision Compatibility
Seamlessly integrates with standard transfer cassettes, eliminating gel-membrane misalignment risks.
Pre-soaking in transfer buffer (10–15 mins) maximizes sponge expansion for tight "sandwich" assembly.
Durability & Cost-Effectiveness
Resists degradation from common lab solvents, supporting 5–10 reuses with proper care (rinse with distilled water, air-dry).
Applications
High-throughput protein analysis in cancer research, neuroscience, and immunology.
Transfer of high molecular weight proteins (e.g., titin, dystrophin).
Multiplex Western blotting requiring minimal background noise.
Usage Guidelines
Standard "Sandwich" Assembly:
With the transfer cassette's black side facing down, layer in this order:
Sponge (pre-soaked in buffer) → Filter paper → Gel → NC/PVDF membrane → Filter paper → Sponge
Use 1 sponge per side and close the cassette under uniform pressure.
Storage and Transportation
Transfer Sponge Should be stored and transported at room temperature, preventing moisture, and it remains effective for a long time.
Operational Safety:
Strictly adhere to laboratory safety protocols:
Wear a lint-free lab coat to prevent cross-contamination
Use nitrile or latex disposable gloves to avoid direct skin contact with chemicals or biological agents
Discard used sponges as biohazard or chemical waste, following institutional disposal guidelines
Handling Precautions
Avoid prolonged contact with solvents or acidic solutions, as these may accelerate material degradation. For cleaning between uses, gently rinse with distilled water and air-dry fully before reuse. Store the sponge separately from reactive substances to prevent unintended interactions.
