MQAE Fluorescent Chloride Lon Detection Kit

MQAE is a 6-methoxyquinolinium derivative. Compared with the chloride fluorescence probe SPQ, MQAE has higher chloride sensitivity and fluorescence quantum yield, and it is the most widely used new chloride fluorescence probe. The probe can detect intracellular chloride ions by diffusion-restricted collision quenching. When intracellular chloride ion concentration increases, the fluorescence intensity of MQAE decreases proportionally with the increase of chloride ion concentration.

This kit is based on the above detection principles, mainly MQAE, and provides a special supporting buffer, easy to use, sensitive detection. The dynamic changes of chloride ions in cells can be detected by fluorescence microscopy, flow cytometry or enzyme labeling.

Ship with dry ice; Store at -20℃ away from light for 12 months.

Note: The above kit reaction times correspond to the 96-well plate assay system.

1. Pre-experiment preparation: Cells are inoculated in 96-well plates and pre-treated accordingly to the experimental purpose;

2. Configuration of MQAE working solution: Configure MQAE working solution according to the need, the configuration system can refer to the following table. Note that different cell characteristics are different. During the experiment, the concentration of the probe can be added or reduced according to the actual situation, initial recommended dilution ratio at 1:100;

3. Staining markers (with adherent cells as an example; suspension cell treatment requires centrifugation):

3.1. Remove the original cell culture medium and wash the cells 1-2 times with PBS or other buffers;

3.2. Remove the washing buffer, add 100 μL of MQAE working solution to each well, and incubate for 30 min at 37°C in a cell culture incubator protected from light;

3.3. Remove the MQAE working solution at the end of the incubation and wash the cells 1-2 times using PBS or other buffers;

4. Fluorescence detection: Use fluorescence microscope, enzyme marker and other instruments to carry out its detection. The probe is green fluorescent, EX/EM ≈ 319/462 nm.

1. Due to different intercellular properties and different pre-treatment methods, the ratio of probe use and incubation time range described in the steps are for reference only and can be adjusted according to specific circumstances.

2. Fluorescent dyes have quenching problems, please pay attention to avoid the light operation to slow down the fluorescence quenching.

3. The probe is sensitive to humidity. When used for the first time, please pack it properly to avoid repeated freezing and thawing. MQAE working solution should be dispensed ready to use.

4. For your health and safety, please wear lab coat and gloves during operation.

For Research Use Only!