EdU (5-Ethyl-2′-deoxyuridine)

EdU (5-ethynyl-2'-deoxyuridine) is a thymidine analog containing an acetylene group. EdU can be used for animal in vivo injection. It has no obvious side effects on the organism and has good stability. When it is injected into animals, these small molecules can quickly diffuse into various organs and tissues in animals, and penetrate into cells, where cells proliferate. Instead of thymidine (T), it is incorporated into newly synthesized DNA, and then the target tissue can be prepared into paraffin or frozen tissue sections for detection; EdU is also suitable for the detection of cell proliferation in vitro, suitable for mice, rats and Detection of EdU cell proliferation in various tissues and organs (except blood vessels) of other animal models. The acetylene group in the EdU molecule can undergo a "click" reaction with the fluorescently labeled azide probe under the catalysis of copper ions to form a stable triazole ring, so the newly synthesized DNA can be labeled with the corresponding fluorescent probe.

Compared with the radiolabeled nucleoside incorporation method, the EdU detection method has no limitations such as radioactive contamination; compared with the BrdU detection method, the EdU detection method does not require DNA denaturation, nor does it rely on antigen-antibody reaction, which greatly reduces the complexity of the experiment sex and operating time. EdU cell proliferation assay has been widely used in in vitro cell or tissue cell proliferation assay.

Transportedwith wet ice bags; stored in a dry state at -20°C, valid for 12 months.

1. Centrifuge at low speed before use to ensure that the powder has fully settled to the bottom of the tube before opening the lid;

2. The recommended working concentration for cell proliferation EdU incubation is 1-50 μM, and DMSO can be used to prepare a 10-50 mM stock solution, see Table 1 below;

3. The recommended EdU injection volume for animal modeling is 5 mg/kg (the ratio of EdU injection volume to animal body weight). The actual injection volume and marking time are determined according to the research content and animal conditions. Use PBS or normal saline to prepare the final concentration of 0.5-1 mg/mL.

Table 1 Recommended Preparation Schemeof EdU Stock Solution

1. This product is harmful to human body, so avoid inhaling the dust of this product.

2. This product is recommended to be used together with Click-iT EdU cell proliferation detection kit series (G1601, G1602, G1603, G1604).

3. For your health and safety, please wear a lab coat and gloves when operating.

For Research Use Only!