Prodoct Information
|
Protein full name |
Collagen alpha-1(I) chain |
|
Synonyms |
COL1A1, EDSC, OI1, OI2, OI3, OI4, collagen type I alpha 1, collagen type I alpha 1 chain |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse COL1A1 |
|
Uniprot ID |
P11087 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Extracellular matrix, Cytoplasm |
|
Predicted MW. / Observed MW. |
138 kDa / 120-140 kDa/180-250 kDa |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
skin, uterus, lung, cartilage |
|
IHC/IF |
Human, Mouse, Rat |
1: 200-1: 1700 |
lung, skin |
Background
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
Images
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WB analysis of CollagenI(GB11022). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
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IHC analysis of CollagenI(GB11022). Sample: Mouse lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Primary antibody: 1: 1700, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of CollagenI(GB11022). Sample: Rat skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Primary antibody: 1: 1700, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
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IHC analysis of CollagenI(GB11022). Sample: Rat lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37℃,30min. Primary antibody: 1: 1700, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
