Anti-Collagen II Rabbit PAb

Protein full name

Collagen alpha-1(II) chain

Synonyms

COL2A1, ANFH, AOM, COL11A3, SEDC, STL1, collagen type II alpha 1, collagen type II alpha 1 chain

Immunogen

Recombinant protein corresponding to Mouse COL2A1

Uniprot ID

P28481

Isotype

IgG

Purity

Affinity purification

Subcellular location

Secreted, extracellular space, extracellular matrix

Predicted MW. / Observed MW.

142 kDa / 180-200 kDa

WB

Human, Mouse, Rat

1: 500-1: 2000

fibrotic liver, cartilage, skin

IHC/IF

Mouse, Rat

1: 100-1: 500

bone

WB analysis of CollagenII (GB11021).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 1000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

IHC analysis of collagen II (GB11021).

Sample: Rat bone (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: , 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

IF analysis of collagen II (GB11021).

Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: , 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

IF analysis of collagen II (GB11021).

Sample: rat bone (Paraffin), 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.

Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.

Primary antibody: , 4℃ overnight.

Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.