Prodoct Information
Product Information
|
Protein full name |
Actin, cytoplasmic 1 |
|
Synonyms |
ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse beta-Actin |
|
Uniprot ID |
P68369 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. / Observed MW. |
42 kDa / 45 kDa |
Applications
|
Applications |
Species |
Dilution |
Positive Sample |
|
WB |
Human, Mouse, Rat |
1: 1000-1: 2000 |
lung, kidney, brain |
|
IHC/IF |
Human, Mouse, Rat |
1: 500-1: 1000 |
colon cancer, liver |
|
ICC/IF |
Human, Mouse, Rat |
1: 300-1: 1000 |
NIH/3T3, A549, etc. |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility .
Images
|
|
WB analysis of beta Actin (GB11001). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 5000, RT, 1h.
|
|
|
IF analysis of beta Actin (GB11001). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
|
Storage |
Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
|
Storage Buffer |
PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
