Description
Technical Parameters
Prodoct Information
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Protein full name |
Proliferating cell nuclear antigen |
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Synonyms |
PCNA, ATLD2, proliferating cell nuclear antigen |
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Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse PCNA |
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Uniprot ID |
P17918 |
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Isotype |
IgG |
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Purity |
Affinity purification |
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Subcellular location |
Nucleus |
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Predicted MW. / Observed MW. |
29 kDa / 36 kDa |
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Applications |
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WB |
Human, Mouse, Rat |
1: 1000-1: 2000 |
HeLa, SW480, B16 |
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IHC/IF |
Human, Mouse, Rat |
1: 200-1: 1000 |
intestinal cancer, liver cancer, lung cancer |
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ICC/IF |
Human, Mouse, Rat |
1:200-1: 400 |
HeLa |
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IP |
Human, Mouse, Rat |
1: 50-1: 200 |
HeLa cells |
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Storage |
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Storage |
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
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Storage Buffer |
PBS with 0.02% sodium azide, 100 μg/mL BSA and 50% glycerol. |
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Background |
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| Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics. | |||
| Images | |||
 Western blot analysis of PCNA (GB11010) at dilution of 1: 2000
 Immunohistochemistry analysis of paraffin embedded human lung cancer using PCNA (GB11010) at dilution of 1: 500
 Immunofluorescence analysis of paraffin embedded human intestinal cancer using PCNA (GB11010) at dilution of 1: 500
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