Western Protein Marker Ⅱ (Exposure)

The Western Protein Marker II is a prestained, visualizable mixture of 7 high-purity recombinant proteins and 3 prestained recombinant proteins. The bands display in Tris-Glycine gel range from 30-209 kDa (~30, 38, 50, 62, ~70, 78, ~105, ~113, 154, 209 kDa), of which 70 kDa is the red pre-stained band, 30 kDa and 105 kDa are the blue pre-stained bands to monitor of electrophoresis and transfer efficiency. The seven recombinant proteins (38, 50, 62, 78, 113, 154, 209 kDa) can bind almost all types of antibodies (except chicken antibodies) and can bind antibodies simultaneously with the target protein and can be colourised by ECL or other means.

1. 70 kDa is a rose red pre stained band, and 30 and 105 kDa are blue pre stained bands, which are convenient for observing the electrophoresis status, determining the membrane transfer effect and direction;

2. Seven Marker bands can be colored together with the target protein, facilitating timely determination of the molecular weight of the target protein after color development (without the need for classical methods to label and compare protein molecular weight standard bands on the membrane);

3. Seven Marker bands are not labeled or coupled to other molecules, indicating more accurate protein molecular weight.

Ship with wet ice; Store at -20℃, valid for 12 months.

1. This product is ready to use with no heating, diluting or additional reducing agent necessary. Thaw at room temperature and mix gently and thoroughly.

2. It is suggested that qualified laboratories can determine the appropriate sample volume by their own experimental conditions and habits when using this product for the first time, which can not only save costs, but also obtain better experimental figures (For primary antibodies of mouse origin, 5 μL of this product is recommended for simultaneous protein electrophoresis with the sample; for primary antibodies of rabbit origin, 5 μL of this product is recommended for simultaneous protein electrophoresis with the sample after 4-fold dilution in the Dilution Buffer provided).

3. Prestained Protein Marker II should be stored at -20℃ immediately after use.

1. Do not boil the protein marker, which can lead to degradation or decolourisation of protein bands

2. If the Western Protein Marker has a thicker band, it can be used after appropriate dilution with Dilution Buffer, which will weaken the colour of the three pre-stained protein bands in the Marker.

3. Due to the uneven specificity of antibodies on the market, it is normal to encounter the development of pre-stained bands, which can be clearly distinguished according to the size of the location;

4. This prestained protein marker can be stored at room temperature for ≥20 days; store at 2-8℃ for ≥20 months; store at -20℃ for ≥12 months.

5. For your safety and health, please wear safety glasses, gloves, or protective clothing.

Figure 1. Band profile of Western Protein Marker Ⅱ

For research use only!