Product Introduction
|
Product Name |
Cat.No. |
Spec. |
|
5× SDS-PAGE Loading Buffer (Reduced) |
G2013-1ML |
1 mL |
|
G2013-100ML |
100 mL |
Description
The 5×SDS-PAGELoading Buffer (Reduced), containing Tris-HCl buffer (63 mM, pH6.8),DTT (20 mM),glycerol (10%), SDS (2%) and bromophenol blue (0.0025%), is used for electrophoretic loading of reduced SDS-PAGE protein samples.
Storage and Handling Conditions
Product shipped at wet ice, stored at -20℃, valid for 12 months.
Component
|
Component |
G2013-1ML |
G2013-100ML |
|
5×SDS-PAGELoading Buffer (Reduced) |
1 mL |
100 mL |
|
Protocol |
1 |
|
Assay Protocol/Procedures
1. Mix each 4μL protein sample with 1μL 5×SDS-PAGELoading Buffer (reduced sample).
2. Heat in a metal bath or boiling water bath for 3-5 minutes to fully denature the protein sample.
3. It can be added to the spotting well after cooling to room temperature.
Note
1. The product should be returned to room temperature before used.
2. Please wear lab coat and disposable gloves during operation.
For Research Use Only!
