Product Introduction
|
Product Name |
Cat. No. |
Spec. |
|
30% Acrylamide-Bisacrylamide (29:1) |
G2004-100ML |
100 mL |
|
G2004-500ML |
500 mL |
Product Description/Introduction
30% acrylamide (29:1) is an aqueous solution containing 30% acrylamide, with a ratio of acrylamide to methyl-diacrylamide is 29:1. It is commonly used for the preparation of PAGE gels and SDS-PAGE gels for the separation of protein or nucleic acid, and is a common stock solution used in molecular biology laboratories for gel preparation.
Storage and Shipping Conditions
Ship with wet ice; Store at 2-8℃ away from light, valid for 12 months.
Production components
|
Component |
G2004-100ML |
G2004-500ML |
|
30% Acrylamide-Bisacrylamide (29:1) |
100 mL |
500 mL |
|
Manual |
1 pc |
|
Assay Protocol/Procedures
1. For the selection of protein molecular weight and gel concentration, please refer to the following table：
|
Protein molecular weight range (kDA) |
Suitable gel concentration ( % ) |
|
＜10 |
15-20 |
|
10-40 |
12-15 |
|
40-100 |
10-12 |
|
100-500 |
5-10 |
2. According to the molecular weight of the target protein, the concentration of the separation gel can be selected and prepared according to the following table:
|
Separation gel concentrations ( % ) |
8% |
10% |
12% |
15% |
18% |
20% |
|
H2O /mL |
4.63 |
3.97 |
3.3 |
2.3 |
1.3 |
0.63 |
|
30% Acr-Bis (29:1) /mL |
2.67 |
3.33 |
4.0 |
5.0 |
6.0 |
6.67 |
|
1.5M Tris-HCl (pH 8.8) /mL |
2.5 |
2.5 |
2.5 |
2.5 |
2.5 |
2.5 |
|
10%SDS /μL |
100 |
100 |
100 |
100 |
100 |
100 |
|
AP (Ammonium Persulfate) /μL |
100 |
100 |
100 |
100 |
100 |
100 |
|
TEMED/μL |
5 |
5 |
5 |
5 |
5 |
5 |
|
Total volume * / mL |
10 |
|||||
* The total volume does not include the TEMED volume.
3. The preparation of stacking gel can refer to the following table:
|
Stacking gel concentration (%) |
5% |
|||
|
H2O /mL |
1.93 |
2.89 |
3.86 |
5.79 |
|
30% Acr-Bis (29:1) /mL |
0.5 |
0.75 |
1.0 |
1.5 |
|
1.0M Tris-HCl (pH 6.8) /mL |
0.5 |
0.75 |
1.0 |
1.5 |
|
10%SDS /μL |
40 |
60 |
80 |
120 |
|
AP (Ammonium Persulfate) /μL |
30 |
45 |
60 |
90 |
|
TEMED/μL |
4 |
6 |
8 |
12 |
|
Total volume * / mL |
3 |
4.5 |
6 |
9 |
* The total volume does not include the TEMED volume.
Note
1. This product has certain toxicity, please pay attention to protection when use.
2. Temperature has an effect on the solidification time of gels. Generally, the lower the temperature, the longer the solidification time; the higher the temperature the faster the gel.
3. The gel needs sufficient solidification time, and it is recommended that the gel be fully prepared and left to ensure that the it gels thoroughly.
4. If you need a full set of reagents, you can buy other products of our company, such as SDS-PAGE gel preparation kit (G2003/G2037), or super-fast color gel preparation kit series (G2041 / G2042 / G2043 G2044 / G2045 / G2060 / G2061 / G2062 / G2063 / G2064), and high resolution ultra-fast color gel preparation kit series (G2066/G2067/G2068, G2071/G2072/G2073), can meet different experimental needs.
5. For your safety and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
