Inorganic Pyrophosphatase (Thermostable)

Product Name

Cat. No.

Spec.

Inorganic Pyrophosphatase (Thermostable)

G3421-200U

200 U

Product Description:

Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4 +H2O+PPase → 2HPO4-2). The enzyme requires a divalent metal cation, with Mg2+ conferring the highest activity. This product exerts its effect at 16-37℃, showing the strongest effect at 25℃. The Inactivation temperature is above 65℃.

Applications:

l High yield RNA synthesis by in vitro transcription.

l DNA polymerization reactions: preventing accumulation of pyrophosphate.

l Removal of contaminant PPi in reagents used for SNP genotyping by methods based on the detection of pyrophosphate.

Source:

An E. coli strain carrying a plasmid encoding a pyrophosphatase from the extreme thermophile Thermococcus litoralis.

Purity:

≥95% by SDS-PAGE

Endogenous nucleic acid

＜1 pg/μL by qPCR

Concentration:

2 U/μL

Definition of Activity Unit:

One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 100 mM Tris-HCl [pH 8.0], 2 mM MgCl2, 2 mM PPi, reaction volume of 0.5 ml).

Storage (Dilution) Buffer:

20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 8.0.

Inactivation or inhibition

l Inhibitors: imidodiphosphate, α ω glycol disphosphates, methanedial diphosphate, 1,2-ethanedial diphosphate. ·

l Inactivation by heating is not complete, reliably removed by spin column or phenol/chloroform extraction.

Storage Conditions:

Store at –20°C up to 12 months.

Component Number

Component

G3421-200U

G3421-1

Inorganic Pyrophosphatase (Thermostable)

100 μL

Manual

One copy