MagBind Serum/Plasma Free DNA Extraction Kit

 

Product Name	Cat.No.	Spec.
MagBind Serum/Plasma Free DNA Extraction Kit	G3612-50T	50T

 

 

MagBind Serum/Plasma Free DNA Extraction Kit utilizes the reversible adsorption of nucleic acids by superparamagnetics nano beads, coupled with a unique buffer system, can efficiently bind smaller DNA fragments (50~500 bp) and minimize the binding of larger fragments such as genomic DNA. This product can quickly and reliably isolate free DNA from a small amount of cell-free body fluids such as fresh or frozen serum, plasma and urine, and the isolated products can be used for PCR amplification, sequencing and genotyping and other downstream related experiments.

 

 

Proteinase K and Carrier RNA are shipped with wet ice and stored at -20°C. Other reagents are shipped and stored at room temperature; valid for 12 months.

 

 

Component Number	Component	G3612-50T
G3612-1	Buffer GBL	20 mL
G3612-2	Buffer GPD	14 mL (add 18 mL of anhydrous ethanol before use)
G3612-3	Buffer GPW	14 mL (add 56 mL of anhydrous ethanol before use)
G3612-4	Proteinase K	1 mL
G3612-5	Carrier RNA	50 μL
G3612-6	SweMag Beads	1.5 mL
G3612-7	Buffer TE	10 mL
Manual		One copy

 

 

1. Anhydrous ethanol and 50 mL Nuclease-free centrifuge tube are required but not supplied in this kit.

2. Add all the RNase A provided in the kit to Buffer P1 before use, and it can be stored at 2~8°C for 6 months.

3. Please add 75 mL anhydrous ethanol to Buffer PD and 163 mL anhydrous ethanol to Buffer PW before use.

4. If Buffer P2 precipitates, please heat it in a water bath at 37°C for a few minutes to restore clarification. After using Buffer P2, the lid should be closed immediately to avoid long-term contact with air.

5. Please pre-cool Buffer P3 at 4°C or ice bath before use.

 

 

1. If Buffer GBL precipitates, please heat at 37℃ to dissolve and use it after returning to room temperature.

2. Please add 18 mL anhydrous ethanol to Buffer GPD and 56 mL anhydrous ethanol to Buffer GPW, mix it thoroughly before first use.

Assay Protocol / Procedures

1. 200 μL of serum, plasma, urine and other samples were taken into a 1.5 mL centrifuge tube, bring the volume up to 200 μL with PBS or Nuclease-free Water if the sample volume is less than 200 μL.

2. 400 μL of Buffer GBL, 20 μL of Proteinase K and 1 μL of Carrier RNA were added to the centrifuge tube, and the samples were thoroughly mixed and incubated at 56℃ for 10 min. Mix 2-3 times in reverse during this period.

3. Add 500 μL of isopropanol, invert and mix well, then add 30 μL of SweMag Beads (Swirl until evenly dispersed before use).

4. After vortex mixing, leave it at room temperature for 10 min. During this period, use a pipette or vortex to mix it for 2-3 times until the magnetic beads are evenly dispersed.

5. Place the tube on a magnetic stand and left to stand for 30 s until the magnetic beads were fully adsorbed. Gently reverse the centrifuge tube with the magnetic stand several times to wash the residual magnetic beads off the tube wall. When the supernatant is clear, aspirate and discard the supernatant (To avoid affecting the extraction efficiency, do not disturb the SweMag Beads).

6. Remove the magnetic stand, add 600 μL of Buffer GPD (check whether anhydrous ethanol has been added before use), and mix it gently with a pipette until the magnetic beads are evenly dispersed. Place the tube on a magnetic stand and left to stand for 30 s. Gently reverse the centrifuge tube with the magnetic stand several times to wash the residual magnetic beads and salt off the tube wall. When the supernatant is clear, aspirate and discard the cleared supernatant. (To avoid affecting the extraction efficiency, please be sure to absorb all residual liquid in the centrifuge tube).

7. Remove the magnetic stand. Add 600 μL of Buffer GPW (check whether anhydrous ethanol has been added before use), and mix it gently with a pipette until the magnetic beads are evenly dispersed. Place the tube on a magnetic stand and left to stand for 30 s. Gently reverse the centrifuge tube with the magnetic stand several times to wash the residual magnetic beads and salt off the tube wall. When the supernatant is clear, aspirate and discard the cleared supernatant (To avoid affecting the extraction efficiency, please be sure to absorb all residual liquid in the centrifuge tube).

8. Repeat step 7.

9. Open the cap of the centrifuge tube and leave the tube at room temperature for 5-10 minutes to allow the ethanol to evaporate completely (Avoid excessive drying of the magnetic beads, which may affect the nucleic acid yield);

10. Remove the magnetic stand, add 30-50 μL of Buffer TE or Nuclease-free Water and mix it gently with a pipette until the magnetic beads are evenly dispersed. Leave it at room temperature for 5 min.

11. Place the centrifuge tube on a magnetic stand until the magnetic beads are fully adsorbed and aspirate the supernatant into a new Nuclease-free centrifuge tube to obtain high purity free DNA.

 

 

1. The sample should avoid repeated freezing and thawing, and can be frozen at -80℃ for long-term storage.

2. There may be magnetic bead residue during elution and inhalation of magnetic bead should be avoided when sampling.

3. Please read the instructions carefully before use, and strictly follow the instructions. Clinical samples should be carried out in the biosafety cabinet. If the processed samples contain pathogenic live viruses, etc., it should comply with the requirements of the relevant national laboratory biosafety regulations.

 

For Research Use Only!