Serum/Plasma Free DNA Extraction Kit

This kit utilizes the reversible adsorption of nucleic acids by adsorption column, coupled with a unique buffer system, can efficiently bind smaller DNA fragments (50-500 bp) and minimize the binding of larger fragments such as genomic DNA. This product can quickly and reliably isolate free DNA from a small amount of cell-free body fluids such as serum, plasma and urine, and the isolated products can be used for PCR amplification, sequencing and genotype and other downstream related experiments.

Proteinase K and Carrier RNA shipped with wet ice and stored at -20°C. Other reagents were shipped and stored at room temperature; valid for up to 12 months.

Before starting (please read carefully).

1. If Buffer CBL precipitates, please heat at 37°C to dissolve and use it after returning to room temperature.

2. Please add 17 mL anhydrous ethanol to Buffer CPD and 56 mL anhydrous ethanol to Buffer CPW, mix it thoroughly before first use.

1. 200 μL of serum, plasma, urine and other samples were taken into a 1.5 mL centrifuge tube, bring the volume up to 200 μL with PBS or Nuclease-free Water if the sample volume is less than 200 μL;

2. 20 μL Proteinase K,200 μL Buffer CBL, and 1 μL Carrier RNA were added to the centrifuge tube, and the samples were thoroughly mixed and incubated at 56℃ for 10 min. Shake the sample from time to time;

3. Add 400 μL isopropanol, invert and mix well, leave it at room temperature for 5 min, Brief centrifugation;

4. Transfer the solution from the previous step to the DNA Spin Column, centrifuge at 12000 rpm for 1 minute, and discard the filtrate from the Collection tube;

5. Add 500μL Buffer CPD into DNA Spin Column (check whether anhydrous ethanol has been added before use), centrifuge at 12,000 rpm for 1 min, and discard the filtrate from the Collection tube;

6. Add 600 μL Buffer CPW into DNA Spin Column (check whether anhydrous ethanol has been added before use), centrifuge at 12,000 rpm for 1 min, and discard the waste liquid;

7. Repeat step 6 for a second 600 μL Buffer CPW wash step;

8. Put the DNA Spin Column on the Collection tube and centrifuge it at 12,000 rpm for 2 min;

9. Transfer DNA Spin Column into a new Nuclease-free 1.5mL centrifuge tube, open the lid and leave it at room temperature for 3-5 min to make the residual ethanol of DNA Spin Column completely volatilize;.

10. Add 30-50 μL Buffer TE or Nuclease-free Water to the center of the DNA Spin Column and left at room temperature for 2-5 min, centrifuge at 12,000 rpm for 2 min to elute free DNA. To increase the yield, the first eluent can also be added back to the DNA Spin Column, left at room temperature for 2min, centrifuge at 12,000 rpm for 2 min, and collect again.

1. The sample should avoid repeated freezing and thawing, and can be frozen at -80℃ for long-term storage.

2. Please read the instructions carefully before use, and strictly follow the instructions. Clinical samples should be carried out in the biosafety cabinet. If the processed samples contain pathogenic live viruses, etc., it should comply with the requirements of the relevant national laboratory biosafety regulations.

For Research Use Only!

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