McCoy’s 5A (modified) Medium, Powder

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Different from other media, McCoy's 5A contains the reducing glutathione, bacto-peptone, a high level of glucose, and also includes Hanks's salts to enable use outside a CO2 incubator. McCoy's 5A medium modified generally supports the propagation of many types of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung rat embryos and other tissues. It can also be used for growth of established cell lines and explants from biopsy tissues.

This product isprovided as powder,contains 16.67 mM D-glucose,1.5 mM L-alanyl-L-glutamine, phenol red indicator,without sodium pyruvate,without HEPES. The powder forms of culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocols below for details)

Ship at room temperature; Store away from light at 2-8℃, valid for36 months.

1. Please use sterile solutions of 1 N NaOH or HCl prepared by membrane iltration using appropriate ilters.

2. Filter using a low protein binding ilter.

3. Keep the container with medium covered during stirring and iltration.

4. Do not over-mix. Stop stirring when the product is completely dissolved.

1. To a mixing container that is as close to the final volume as possible, add 950 mL of distilled water.

2. Add McCoy's 5A (modified) medium, powder g to room temperature (15℃to 30℃) water (recommed WFI for cell culture or distilled water) with gentle stirring. Do not heat the water.

3. Rinse the inside of package to remove all traces of powder.

4. Add sodium bicarbonate (2.2 g/L) to the medium.

5. Adjust the pH to 0.2-0.3 units below the desires final working pH by slowly adding, with stirring, 1 N NaOH or 1 N HCl. The pH may rise 0.1 to 0.3 units upon filtration.

6. Adjust the final volume with distilled water.

7. Process the medium immediately into sterile containers by membrane filtration with a 0.2-μm filter using a positive pressure system.

For Research Use Only! Not for use in diagnostic procedures!