Rapid Lentivirus Titration Cassette (Colloidal Gold)

 

Product Name	Cat. No.	Spec.
Rapid Lentivirus Titration Cassette (Colloidal Gold)	G1804-10T	10 T

 

 

This product is a semi-quantitative HIV-1 P24 protein assay card based on colloidal gold lateral chromatography that can be used for rapid assessment of the packaging efficiency of relevant lentivirus vectors and pseudoviruses (the P24 protein is the most abundant marker protein in the lentiviral capsid). The product is a rapid detection of free capsid protein P24 secreted into the lentiviral packaging supernatant using colloidal gold immunochromatography as a sensitive, indirect surrogate indicator of viral yield. The detection of capsid protein P24 enables a rapid determination of whether the lentiviral titer in the packaging solution has met the harvest needs. This product requires only 8-12 minutes to obtain results, which is a significant advantage over traditional transfection methods that take 2-3 days.

 

P24 protein concentration in relation to lentivirus titer:

P24 concentration	500 ng/mL	100 ng/mL	50 ng/mL	10 ng/mL	1 ng/mL
LPs/mL	6.25×109	1.25×109	6.25×108	1.25×108	1.25×107
TU/mL	6.25×106-7	1.25×106-7	6.25×105-6	6.25×105-6	6.25×104-5

 

There are approximately 2000 P24 protein molecules in a Lentiviral Particle (LP) and normally 1 TU (Transducing Unit) of infectious virus vector for every 100-1000 LPs; a virus titer of at least 1 x 10⁶ TU/mL is required for certain types of cells to be transfected. (usually concentrated at around 150 times).

 

Result of lentivirus titer fast detection card (colloidal gold method) for 10 min:

 

 

Dilute P24 protein to 500 ng/mL, 100 ng/mL, 50 ng/mL, 10 ng/mL, 1 ng/mL and 0, then drop 80 µ L of diluent into the loading wells, place it flat on the table, stand for 8-12 minutes to observe the results.

 

 

Ship and store at room temperature, valid for 12 months.

 

 

Component Number	Component	G1804-10T
G1804-10T	Rapid Lentivirus Titration Cassette (colloidal gold)	10 T
Manual		1 pc

 

 

1. Open the package and take out the detection card, place it flat on the table.

2. Pipette 80~100 µL of culture supernatant from cells (such as 293 cells) packaged with lentivirus, and drop to the loading wells.

3. Stand for 8~12 minutes to obtain results, and they are invalid after 15 minutes.

 

 

1. In the visual window, both the quality control line (C) and the test line (T) are coloured as positive.

2. The quality control line (C) is colored, the test line (T) is not colored, and the test result is negative.

3. The quality control line (C) is not colored. No matter whether the detection line (T) is colored or not, the result is invalid.

4. The colour of the (T) line varies with the content of P24, the higher the content, the darker the colour; the lower the content, the lighter the colour.

 

 

1. If the color of tissue culture medium causes high background, dilute the sample with PBS or culture medium before chromatography.

2. It is not recommended to freeze the test card.

3. Use as soon as possible after opening.

 

For Research Use Only!