Trypan Blue Stain(Ready-to-use)

Trypan Blue is a reactive dye for cells, which is often used to detect the integrity of cell membranes. Because the envelope of normal living cells is complete, it can repel trypan blue, making it unable to enter the cells, while the permeability of dead cells or cells with incomplete envelope increases, and can be dyed blue by trypan blue. Therefore, the cell survival rate can be determined simply and quickly by trypan blue staining.

This product is trypan blue dye solution with a concentration of 0.2%, which is used for living cell dyeing and counting.

Wet ice transportation; Store at 2-8℃ for 12 months.

1. Collect cells: if they are adherent cells, they should be trypsinized first; Suspend cells to collect the suspension directly. Centrifuge the collected cells at 1000-2000 rpm for 1-3 min, discard the supernatant, and resuspend the cells with an appropriate amount of medium or PBS and other buffers to prepare a single cell suspension.

2. Trypan blue staining: Mix trypan blue staining solution (0.2%) with the resuspended cell sample in a 1:1 ratio. For example, take 10 μL of resuspended cells to a centrifuge tube, add 10 μL of trypan blue staining solution (0.2%), that is, the final concentration of trypan blue is 0.1%, gently pipette and mix, and stain for 3min. 3. Counting: Draw 10μL of stained cells dropwise and add them to the cell counting plate (CCB-6), and use the cell counter (SCC-M630) for automatic counting.

4. Statistics of cell vitality: living cell rate (%)=number of living cells/(number of living cells+number of dead cells) × 100%.

Precautions

1. Trypan blue staining solution is toxic, wear a lab coat and disposable gloves during the experiment.

2. The staining time of trypan blue should not exceed 10min, otherwise it will affect the cell viability.

For Research Use Only!