Product Information
|
Product Name |
Cat. No. |
Spec. |
|
BrdU(5-Bromo-2'-deoxyUridine) |
GC310002-100mg |
100 mg |
Product Description/Introduction
BrdU (5-bromo-2-deoxyuridine) is a thymidine analog that competes with thymidine for incorporation into DNA during the S phase of cell division. When cells in vigorous division are adulterated with BrdU, cells containing BrdU can be detected by using Anti-BrdU (mouse mAb) antibody in Anti-BrdU Detection Kit (G4102) in combination with the corresponding antibody ligase or fluorescein as an indication system. Double labeling by combining with other cellular markers can determine the types of proliferating cells, the rates of proliferation, etc., which is of great significance to the study of cellular dynamics. BrdU is incorporated into newly synthesized DNA in vivo injection or in cell cultures, with accurate positioning that reflects the level of DNA synthesis during the S phase. It is minimally affected by internal or external cellular environments, ensuring that labeled cells are not lost.
Main Parameters
CAS:59-14-3;Molecular formula:C9H11BrN2O5;Molecular mass:307.10;Character:white powder;Purity:≥99.0%.
Storage and Shipping Conditions
Ship with wet ice; Store away from light at -20 °C, valid for 36 months.
Handling Instruction
l Animal Experiment: BrdU powder was dissolved in physiological saline to make a concentration of 10 mg/mL, filtered and sterilized for later use. The dosage administered was generally 50 mg/kg, through intraperitoneal injection, given 4 times with a 2-hour interval. Animals were euthanized 24 hours after the final injection.
l Cell Experiment: The concentration and treatment time of BrdU usage for cells depend on the cell type. Generally, a lower concentration and shorter treatment time are required for tumor cells, while fibroblasts or epithelial cells with slower proliferation require higher concentrations and longer treatment times. It is recommended to use a concentration range of 20-100 μM and a treatment time of 40 min-4 h, which can be adjusted according to the specific cell type. The table below shows commonly used concentrations and times tested for reference purposes only.
|
Cell |
BrdU Concentration (μM) |
Incubation period (min) |
|
A549 |
40 |
40 |
|
Hela |
40 |
40 |
|
NRK-52e |
40 |
40 |
|
SKOV3 |
80 |
120 |
|
H9C2 |
40 |
40 |
Note
1. Please avoid exposure to light when storing and preparing this product. Once prepared, it can be aliquoted and stored at -20℃.
2. We recommend using the G4102 BrdU assay kit for subsequent experiments.
3. For your safety and health, please wear laboratory attire and disposable gloves when handling this product.
For Research Use Only!
