Product Information
|
Product Name |
Cat.No. |
Spec. |
|
DPBS Buffer, Containing Calcium And Magnesium Ions |
G4201-500ML |
500 mL |
Description
DPBS buffer, namely Dulbecco's phosphate-buffered Saline, is a kind of balanced salt solution widely used in biological and biochemical studies, which has a slightly lower Phosphate content than conventional PBS buffer. DPBS IS mainly USED in embryological research, and can BE used as rinse solution, cryopreserved solution and culture medium for embryos and tissues. Glucose and sodium pyruvate can be added to DPBS as needed to maintain the basic nutrition of the culture.
This product contains calcium and magnesium ions and does not contain phenol red.
Ingredients: 2.68mM KCl, 1.47mM KH2PO4, 137mM NaCl, 8.0mM Na2HPO4, 0.9mM CaCl2, 0.49mM MgCl2, pH 7.2-7.8@25℃. The bacteria were removed by 0.1 μm filtration.
Storage and Handling Conditions
Transport and store at room temperature, valid for 24 months.
Component
|
DPBS Buffer Component |
||||
|
Component |
G4200-500ML |
G4201-500ML |
||
|
mg/L |
mM |
mg/L |
mM |
|
|
KCl |
200 |
2.68 |
200 |
2.68 |
|
KH2PO4 |
200 |
1.47 |
200 |
1.47 |
|
NaCl |
8000 |
137 |
8000 |
137 |
|
Na2HPO4•12H2O |
2886 |
8.0 |
2886 |
8.0 |
|
CaCl2 (anhydrous) |
- |
- |
100 |
0.9 |
|
MgCl2•6H2O |
- |
- |
100 |
0.49 |
Note
1. The product is sterilized by 0.1μm filtration and can be used directly.
2. This product contains calcium and magnesium ions, which are easy to produce trace precipitation and can be dissolved after warm water bath. If too much precipitation can not be dissolved, please stop using.
3. Please pay attention to aseptic operation when using this product to avoid contamination.
For Research Use Only!
