Anti-Clumping Agent

In modern bioprocessing, expressing cell lines are increasingly adapted to serum-free or low-serum suspension cultures to achieve higher cell densities and protein yields. However, cellular agglomeration-where cells adhere to form clusters-remains a persistent challenge during adaptation phases and high-density cultivation. While small aggregates initially pose minimal issues, larger clumps lead to nutrient deprivation in inner cells, triggering necrosis. Released cellular debris then compromises the entire culture, exacerbating agglomeration and cell death cycles.

G4105-100ML Cell Anti-Agglomeration Reagent is a chemically defined, protein-free, and hydrolysis product-free solution engineered to mitigate these challenges. Comprising a proprietary blend of dispersing agents and shear-protection components, it provides dual-action efficacy: reducing cell-cell adhesion while shielding cells from mechanical stress during agitation. This results in improved monodispersity, enhanced viable cell densities, and consistent culture performance.

Chemically Defined Formulation: Eliminates variability from undefined components, ensuring batch-to-batch consistency in sensitive processes like CHO or HEK293 cultivation.

Versatile Concentration Range: Adjustable usage (1–4% v/v, recommended 2%) allows optimization for diverse cell types, from fragile insect cells to robust mammalian lines.

Shear Stress Mitigation: Protects cells during high-speed agitation in bioreactors or shaker flasks, preserving membrane integrity and metabolic activity.

Serum-Free Compatibility: Ideal for QbD-driven workflows, enabling seamless integration into perfusion or fed-batch systems without compromising product quality.

Attention: The following protocol is for the addition of conventional exchange solutions, which can also be added directly to the cultured cells during use. The addition volume is the same as the following volume ratio.

1. Add the cell anti-clumping agent to the corresponding cell culture medium, with the addition volume accounting for 2% of the total volume (the amount of anti-clumping reagent required varies under different conditions, usually between 1-4%, 2% is recommended and maybe adjusted.

2. Transfer the suspended cells into a centrifuge tube, centrifugeat800 x g for 3-5 min, remove the original culture medium, and keep the cell precipitate.

3. Resuspend cellswith the working solution containing the cellular anti-colonisation reagent and transfer to the appropriate culture dishes or flasks for incubation.

1. This product is suitable for the domestication of suspension cell system under shaking bed culture conditions, and may not be effective for suspension cells in regular resting conditions.

2. Due to the different properties of different cells, the concentration range described in step is for reference only and can be adjusted according to specific conditions.

3. This product will affect cell transfection, it is recommended to replace it with a normal culture medium without this product before transfection incubation. Add at the time of fluid change after the transfection incubation is complete and does not affect post-transfection expression.

4. For your health and safety, please wear laboratory clothes and gloves when operating.

For Research Use Only!