Ponceau S Staining

Ponceau S staining solution can be used for the rapid detection of proteins on PVDF, nitrocellulose (NC) and cellulose acetate membranes. The principle is that Ponceau S is negatively charged and can bind to positively charged amino acid residues, while Ponceau S can also bind to non-polar regions of the protein, resulting in a red band. The staining of protein by Ponceau S is reversible and can be washed off with distilled water, PBS or other appropriate solution.

The main ingredient of this product is 0.2% Ponceau S.

Ship and store at room temperature, valid for 12 months.

1. The PVDF, nitrocellulose or cellulose acetate membranes are immersed in Ponceau S working solution, and the staining is shaken for 3-5 min or longer until clear bands appeared.

2. Rinse the stained membrane in distilled water for a few seconds to slightly remove the background color until the background is light and the protein band is clear.

3. Rinse with distilled water, PBS or other appropriate solution 2-3 times, 3-5 min each time to remove Ponceau S, and conduct the subsequent Western experiment.

1. Ponceau S staining solution is a ready-to-use product and does not require dilution.

2. Ponceau S staining solution can be recycled and used repeatedly.

3. Ponceau S staining solution is not suitable for protein staining detection on nylon membrane.

4. The kit is easy to use, has a low background and high sensitivity, with a lower limit of detection of 250 ng for proteins.

5. For your safety and health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!