Coomassie Brilliant Blue R250

This product uses Coomassie brilliant blue R250 as dye and does not contain toxic methanol. It is mainly used for routine staining of protein electrophoresis polyacrylamide gels. It can be used as a supplementary reagent for the G2012 Coomassie Brilliant Blue dye kit.

Ship and store at room temperature, valid for 12 months.

Conventional dyeing and decolorization methods:

1. After electrophoresis, remove the polyacrylamide gel and place it in an appropriate amount of Coomassie brilliant blue stain R250 to just cover the gel.

2. Dye 4-12 h at room temperature on a horizontal shaker, or increase the temperature appropriately to reduce the dyeing time.

3. Pour out the stain solution, add appropriate amount of Coomassie brilliant blue decolorization solution to cover the gel, and decolorize for 4-12 h at room temperature until the gel background is clean and clear dark blue protein band can be seen. The decolorization solution should be replaced during the process.

Rapid dyeing and decolorization method：

1. After electrophoresis, remove the polyacrylamide gel and moisten it twice in distilled water, then add appropriate amount of Coomassie brilliant blue dye solution R250 to cover the gel. Heat in the microwave until nearly boiling, then stop heating immediately. If the solution is boiled, the gel will be damaged and broken.

2. Stain on a horizontal shaker for about 5 min at a high temperature until clear protein bands could be seen.

3. Pour out the dye solution, add an appropriate amount of Coomassie brilliant blue decolorization solution (G2022) to cover the gel, and heat it in the microwave oven on low heat until it is close to boiling, then stop heating immediately. Similarly, the boiling solution is easy to lead to gel breakage and fragmentation.

4. The decolorization was carried out on a horizontal shaker for 20-30 min while keeping the decolorizing solution at a high temperature, during which the decolorization solution can be replaced 2-3 times, and the decolorization can be heated and shaken in the microwave oven until the blue background is basically removed and the protein band reaches the expected staining result.

5. After decolorization, the gel can be stored in water or 20% glycerin solution and photographed quickly. If long-term storage is required, dry gel can be prepared.

1. Coomassie Brilliant blue dye R250 can be recycled and used repeatedly.

2. For the glue concentration less than 10%, the operation should be careful to avoid the situation of glue fragmentation.

3. If using rapid dyeing and decolorization method, be sure to use microwave oven for low heat because the staining solution and decolorization solution contain ethanol, stay safe.

4. For your safety and health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!