4-12% Tris SwePAGE Pre-Cast Gel (11-well)

 

Servicebio SwePAGE Pre-cast Gel is a ready-to-use, small-sized polyacrylamide gel that offers convenience and rapid use. The SwePAGE pre-cast gel plate features a unique design with a special surface treatment that enhances protein band resolution, resulting in a more uniform band distribution. The gel in this product is based on the Tris system and is compatible with Tris-glycine SDS-PAGE standard electrophoresis buffer and Tris-glycine SDS-PAGE SWE high-resolution rapid electrophoresis buffer. This product includes two bags of Tris-glycine SDS-PAGE SWE high-resolution rapid electrophoresis buffer powder (G2081-1L powder).

The SWE high-resolution rapid electrophoresis buffer boasts stronger buffering capacity and higher resolution,producing sharper and clearer protein bands.

This product does not contain SDS and can also be used for native electrophoresis with suitable electrophoresis buffers and corresponding reagents.

Compared to traditionally prepared gels in laboratories, SwePAGE pre-cast gel offers the following advantages.

Easy of Use: Ready-to-use, eliminating the need for gel preparation and contact with toxic reagents like monomeric acrylamide.

High Sample Loading Capacity: Maximum sample loading capacity up to 40 μL.

High Resolution: The gel formulation is optimized for the Tris system, providing higher resolution and sharper protein bands.

Excellent Compatibility: Compatible with most small protein gel vertical electrophoresis system (e.g., Servicebio, Liuyi, Bio-Rad, Tanon, etc.).

SwePAGE Pre-cast Gel Plate Dimensions: Width 100 mm, Height 82 mm; Gel Dimensions: Width 85mm, Height 60 mm, Thickness 1.0mm.Different concentrations of isocratic gels including 8%,10%, and 12%, are available for the separation of proteins of different molecular weights. Additionally, three types of gradient gels, namely 4-20%, 4-12%, and 8-16%, are provided to meet the separation needs of proteins within different molecular weight ranges.

 

 

Shipped with wet ice; store at 2-8°C with a shelf life of 12 months.

 

 

1.Dissolve the electrophoresis buffer provided in the kit (Tris-glycine SDS-PAGE SWE high-resolution rapid electrophoresis buffer(G2081) in pure water and adjust the volume to 1 L for use. Remove the SwePAGE pre-cast gel from its packaging and peel off the adhesive tape at the bottom of the gel plate, as shown in Figure 1.

Figure 1: Peel off the tape at the bottom of the gel plate.

 

2.Gently push out the combs from the gel plate,ensuring that the pressure on both sides is consistent as much as possible (refer to Figure 2). Place the gel plate into the gel electrophoresis apparatus.

(Figure 2). Push the comb out of the gel plate

 

3.Preparation before running precast gel electrophoresis: Taking Servicebio vertical electrophoresis equipment (SVE-2) as an example,two seal silicone gaskets are equipped with the equipment,namely the raised section (Figure 3A) and the straight section (Figure 3D). The original seal gasket is generally a raised section, which has a protruding structure on the top. However, the short plate of SwePAGE precast gel has a concave structure, and this part is flat. Therefore, before electrophoresis, the seal silicone gaskets in the electrophoresis tank with a protruding structure need to be removed and reversed, with the flat side facing outward, or directly replaced with straight sections, to prevent electrolytic fluid leakage. The specific steps are as follows: remove the U shaped seal gasket in the electrophoresis tank (Figures 3A, B), reverse the seal gasket, with the flat side facing outward (Figure 3C) orreplace it with a straight section (Figure 3D), and reinsert it into the groove in the frame, as shown in Figure 3, if a Liuyi brand electrophoresis tank is used, the above steps can be ignored. After completing the above steps, place the precast gel plate into the gel electrophoresis apparatus.

Figure 3: Reversing the seal gaskets in the electrophoresis apparatus, changing the seal gaskets.

 

Note: When loading the plastic plate, make sure the shorter edge is facing inward. If only one precast gel is being used, insert a spacer on the other side to prevent electrolyte leakage. This example shows the procedure for reversing the raised seal gasket in our self-developed electrophoresis apparatus. If there is no raised surface, you can skip this step.

4.Pour sufficient 1× SWE high-resolution rapid electrophoresis buffer or Tris-glycine standard electrophoresis buffer into the inner tank of the electrophoresis tank to fill it, and add the same electrophoresis buffer to the outer tank, with the recommended liquid level reaching the middle of the outer tank Notethat for best results,thebufferintheouter tank should nbe addedto a level lower than the inner tank liquid level and should not overflow the gel plate (Note: MOPS and MES electrophoresis buffers are not compatible with the Tris gel system of SwePAGE pre-cast gel. Please use Tris-glycine electrophoresis buffer).

 

5.Use a syringe or pipette to aspirate an appropriate amount of electrophoresis buffer and gently rinse the sample wells to remove bubbles and residual liquid6.Sample Preparation

(1) SDS-PAGE Gel Electrophoresis: Mix the protein sample with 5× SDS-PAGE protein loading buffer (recommended G2013, G2075) in a 4:1 volume ratio, then incubate at 100°C for 3-5 minutes using a metal bath or boiling water bath before use for electrophoresis spotting. The applicable total protein loading amount is 1-100 μg.

(2) Native PAGE Electrophoresis: Mix the protein sample with 5× protein loading buffer without SDS(recommended G2034) in a 4:1 volume ratio. Do not heat; mix well and use immediately for electrophoresis spotting. Note: The electrophoresis buffer provided in the pre-cast gel kit containsSDS, which is not suitable for native electrophoresis.

 

7.Sample Loading and Electrophoresis: When loading the sample, insert the tip of the pipette vertically into the sample well. Be careful not to puncture the gel or deform the gel matrix, as this may result in sample leakage. After completing the sample loading, cover the electrophoresis tank with the lid and insert the power cord into the power socket of the electrophoresis apparatus (red to red, black to black). Perform electrophoresis at a voltage of 200 V for 25-45 minutes, or until the bromophenol blue dye front reaches the bottom of the gel. Note: Adjust the electrophoresis voltage according to the specific

conditions of your experiment. Some electrophoresis apparatuses have poor heat dissipation at the bottom, and using excessively high voltage may cause localized overheating, affecting the electrophoresis results. In such cases, it is necessary to reduce the voltage appropriately and perform the electrophoresis in an ice bath. The table below provides some reference data.

 

Electrophoresis buffer	Voltage	Initial current	Final current	Electrophoresis time
MOPS	200 V	141-156 mA	105 mA	20-30 min
MES	200 V	123-136 mA	79 mA	30-40 min

 

8.After electrophoresis is complete, remove the precast gel from the electrophoresis tank. Carefully insert a pry tool into the gap between the gel plates (as indicated by the red box in Figure 4) and gently pry open the plastic plates from top to bottom until completely separated. The gel may be attached to either side of the gel plates. Remove the plate without the gel, and for the plate with the gel, immerse the gel side into water at an angle and gently lift it. Once the gel drops into the water, remove it from the water for staining or subsequent transfer procedures. Note: Discard the used gel plates and comb as medical waste or laboratory waste. Do not dispose of them in regular household trash bins.

Figure 4: Pry open the gel plates (using one side as an example, repeat this step for the other side)

 

Cat.No.	G2302-10	G2306-10
Name	10% Tris SwePAGE Pre-Cast Gel	4%-12% Tris SwePAGE Pre-Cast Gel
Spec.	10 pcs/box	10 pcs/box
Recommended Separation Range	10-160 kDa	10-300 kDa
Applicable Electrophoresis System	Tris-Glycine	Tris-Glycine
Maximum Sample Loading Volume (μL)	40	40
Sample Wells	11	11
Gel Concentration	0.1	4-12%