Product Introduction
|
Product Name |
Cat. No. |
Spec. |
|
2 x SDS-PAGE Loading Buffer (Reduced) |
G2031-1ML |
1 mL |
Product Description/Introduction
This product is a 2x concentrated protein loading buffer with Bromophenol Blue as the dye for the electrophoretic loading of reduced SDS-PAGE protein samples. It contains Tris-HCl buffer, glycerol, SDS and bromophenol blue and a small amount of reducing agent DTT.
Storage and Shipping Conditions
Ship with wet ice; Store at -20℃, valid for 12 months.
Product Components
|
Component |
G2031-1ML |
|
2× SDS-PAGE Loading Buffer (Reduced) |
1 mL |
|
Manual |
|
Assay Protocol/Procedures
1. Mix well with an equal volume ratio of 2× SDS-PAGE Loading Buffer (reduced) to protein sample.
2. Heat in a metal or boiling water bath for 3-5 minutes to fully denature the protein sample.
3. Cool to room temperature and load on the gels.
Note
1. This product contains a small amount of the reducing agent DTT, has a slightly pungent odor, but does not contain mercaptoethanol. Please pay attention to protection when use.
2. Make sure the product is fully re-warmed before use.
3. For your safety or health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
