Hematoxylin Differentiate Solution

Differentiation refers to the process of removing the excessive binding dye from the tissue with some specific solutions after tissue staining. This process is differentiation, and the solution used is called differentiation solution. In HE staining, low concentration of hydrochloric acid is often used as the differentiation solution, because the acid can destroy the quinone structure of hematoxylin, and make the pigment separate from the tissue and fade. After hematoxylin staining, tissues must be differentiated to remove excessive binding and non-specific adsorbed dyes before eosin staining can be carried out to ensure distinct color development between nuclei and cytoplasm.

This product is hematoxylin differentiation solution, used for hematoxylin differentiation after staining, mainly composed of dilute acid solution.

Store and transport at room temperature, valid for 18 months.

Take HE staining as an example:

1. Hematoxylin staining: The dewaxed sections are stained with hematoxylin stain solution for 3-5 minutes and washed with tap water.

2. Differentiation: The sections were fully rinsed with hematoxylin differentiation solution for 2-5 s,tap water running water to rinse well.

3. Back to blue: Hematoxylin back to blue solution for 2-5 s, tap water running water to rinse well.

4. Eosin staining: the sections were dehydrated by 85% and 95% gradient ethanol for 5 min each. Then it was stained in eosin (alcohol-soluble) for 5 min, and dehydrated twice with absolute ethanol for 5 min each.

5. Transparent: The slices are then dehydrated with fresh absolute ethanol for 5 min, the xylene is transparent for 5 min, and the fresh xylene is replaced with transparent for 5 min.

6. Mounting: Add neutral gum dropwise for mounting.

1. The differentiation time should be adjusted appropriately according to the thickness of the section and the tissue category.

2. For your safety and health, please wear a lab coat and disposable gloves when operating.

For Research Use Only!