Product Introduction
|
Product Name |
Cat.No. |
Spec. |
|
Aniline Blue Stain (10%) |
G1071-100ML |
100 mL |
Product Description/Introduction
This product is a 10% aniline blue aqueous solution, which can be used to display fungi in plant tissues, and can also be used to dye the morphological structure of plant tissues. After staining, the fungal hyphae were blue filamentous, and the tissue background was light blue.
Storage and Handling Conditions
Store and transport at room temperature; valid for 6 months.
Assay Protocol
1. Dewaxing the paraffin slices to water: (This step is for reference only) Put the slices into xylene I for 20 min - xylene II for 20 min - absolute ethanol I for 5 min - absolute ethanol II for 5 min - 75% ethanol for 5 min, and wash them with tap water.
2. Aniline blue staining: dye the slices with aniline blue dye (10%) for 5-10 min, wash them quickly with tap water, and bake the slices at 60 ℃.
3. Dehydration seal: the slices are transparent with xylene for 5 min, and sealed with neutral gum.
Note
1. This product can be reused several times and should be kept sealed to prevent evaporation of the active ingredient.100 mL of staining solution can be used for staining (immersion staining) of approximately 500 sections. When the tissue or cell coloring is significantly lighter or the color is abnormal, please replace with new staining solution.
2. Please wear a lab coat and disposable gloves during operation.
For Research Use Only!
