Congo Red Dye Solution

Amyloid substance is an extracellular eosinophilic substance with no fixed shape, mainly composed of proteins, most of which are arranged in reverse β- Folding layer structure, such amyloid substance can exist in different tissues and organs, and the disease is called amyloidosis. Congo red can be used for coloration of amyloid in living and tissue sections. The basic principle is that amyloid has a high affinity for Congo red dye. Its hydroxyl group combines with the amino group of Congo red to make amyloid red. Congo red staining is a classical method of amyloid staining.

This product is composed of Congo red dye solution and alkaline differentiation solution, which can be used to dye amyloid substances. After staining, the amyloid plaque material presents light pink to orange red cloud or patch shape, and under polarized light, the amyloid material also presents yellow green double refraction.

Store and transport at room temperature; valid for 18 months.

If it is necessary to re stain the nucleus, prepare hematoxylin staining solution, hematoxylin differentiation solution and hematoxylin blue returning solution. Prepare ethanol, xylene, neutral gum, etc.

1. The paraffin section shall be dewaxed and rewatered according to the routine steps.

2. Congo red staining: slice into Congo red dye solution for overnight impregnation, and then wash with tap water for 2 minutes.

3. Differentiation: The slices were differentiated into positive amyloid plaques with obvious background after alkaline differentiation solution, and then washed with tap water.

4. (Optional) Nucleation staining: the slices were stained with hematoxylin dye solution (G1004) for 1 min, differentiated with hematoxylin differentiation solution (G1039), washed with tap water, and the hematoxylin bluing solution (G1040) turned blue, washed with running water.

5. Dehydration and mounting: slice is dehydrated by anhydrous ethanol for three times, each time for 5 minutes; the xylene is transparent twice for 5min each time. Drop a neutral gum to mount the slide.

1. Alkaline differentiation solution should be sealed to prevent solvent volatilization from affecting differentiation effect. Use it as soon as possible after opening.

2. The differentiation time varies according to section thickness and tissue type. After differentiation, the differentiation should be terminated by washing in time to prevent excessive differentiation, and sufficient washing should be ensured to completely remove the differentiation solution.

3. Each set of staining solution can be used for staining (dip-staining) approximately 90 sections. Replace the stain with a new one when the tissue or cell coloring is significantly lighter or abnormal.

4. Wear a lab coat and disposable gloves during operation.

For Research Use Only!