Special Fixative Solution For IEM

The ultrastructure of tissue cells can be observed by transmission electron microscopy, and the distribution of antigens (antibodies) in tissues can be detected by immunohistochemistry with labeled specific antibodies (or antigens) and observed by ordinary light microscopy. Immune electron microscopy (sem) technology is the electron technology and the product of chemical technology, according to the principle of the height of the antigen antibody specificity combined, with high electron density of markers (such as gold, iron, protein, etc.) from the ultrastructure level detection some antigenicity substance location, in other words, the immune electron microscopy (sem) was observed under transmission electron microscopy (sem) immunohistochemical detection result of ultrastructure level, It's an extension of Immunofluorescence microscopy. One of the most important problems in immunoelectron microscopy is the choice of fixation solution, which requires the preservation of the antigenicity of tissue components and the preservation of the ultrastructure of cells.

This product is a special fixative for immunoelectron microscopy, which is specially used for the fixation of tissue samples in the early stage of immunoelectron microscopy. It can preserve the ultrastructure and antigen of tissues well. The main active ingredients of the product are formaldehyde and glutaraldehyde.

Wet ice transportation; Stored at 4℃ away from light, valid for 24 months.

G1124

  Special Fixative Solution for IEM 100 mL  

For tissue samples, the special fixation solution for immunoelectron microscopy was loaded in Petri dishes in advance. When taking materials, use a sharp blade to cut the target tissue accurately and immediately immerse in the fixing solution. Continue cutting into 1 mm³ sizes with a scalpel in fixation fluid. Then, the cut small tissue block was transferred to the EP tube filled with fresh fixative for fixation at room temperature and protected from light for 2h, after which they were transferred to 4°C for storage and transportation protected from light. For adherent cells, discard the culture medium for the cultured cells and the fixative was restored to room temperature, the cells were added to the Special Fixative Solution for IEM and fixed at room temperature and protected from light for about 5 min. Cells were carefully scraped by cell scraping, and cell precipitates were collected by low-speed centrifugation, and then fixative was added to soak cell precipitates at room temperature for 30 min, and then transferred to 4°C for storage and transportation away from light. If there is no requirement for cell morphology, the cultured cells can be digested into cell suspension with trypsin and then fixed according to the procedure of cell suspension. For suspended cells: the cultured cells were centrifuged at low speed and the medium was discarded. Add the Special Fixative Solution for IEM to restore room temperature and fix it at room temperature and avoid light for 30 min, and then turn to 4℃ to avoid light for storage and transportation.

1. For immunoelectron microscopy detection, sample sampling is critical in the early stage, which requires skillful technique and rapid sampling. The sampling and fixation of the target position should be completed within 3 minutes. The mechanical damage such as stretching, contusion and extrusion to the tissue should be minimized during the sampling. The tissue sampling volume should be as small as possible, not more than 1 mm³. If the tissue block is too large, the internal tissue cannot be fixed timely and adequately, which may affect the ultrastructure.

2. The fixed samples need to be stored and transported at 4℃ to avoid crystallization of the fixative caused by too low temperature.

3. Use the device in a well-ventilated environment with proper protection.

4. Please tighten the cap in time after use to prevent volatile active ingredients.

5. Wear a lab coat and disposable gloves during operation.

For Research Use Only!