Carnoy's Fixative Solution (Used To Fix Intestinal Mucus)

In histopathology, the tissue must be promptly and effectively fixed before subsequent embedding and sectioning can be completed. Organization fixed role is to maintain the inherent morphology and structure of organization, cell, prevent bacteria corrosion and tissue autolysis, save the inherent material inside the cell, the cell protein coagulation, as far as possible to reduce or end of endogenous and exogenous enzyme reaction, keep the cell or tissue basically like living material, also can make the organization hardening, easy to repair the cut of the organization. For certain infectious specimens, can prevent the spread of disease; Preserve the gross specimen. Finally, fixation can enhance the effect of staining.

This product (Carnoy's Fluid/ Carnoys Fixative) can be used to fix the mucus layer of animal intestinal tissue, and the sample can be used for PAS and AB-PAS staining of intestinal mucus layer to obtain excellent staining effect. This product composition: ethanol: glacial acetic acid =3:1.

Transport at room temperature; Store in a cool and dry place, away from direct sunlight, valid for 24 months.

When taking animal intestinal tissue, the length of intestinal tissue is required to be greater than 5 mm, do not wash with water after taking the material, immediately put into the fixative solution, the fixation time is more than 4 h, not more than 7 days, which can better preserve the intestinal mucus layer. After the tissue is fixed, short-term dehydration (absolute ethanol twice, 40-60 min each time) is directly used with absolute ethanol, xylene is transparent, paraffin embedding, paraffin sections are prepared, and then PAS, AB-PAS is used to stain the mucus.

1. Use the product in a well-ventilated environment with proper protection to avoid inhalation.

2. Intestinal tissue should not be washed during sampling, and intestinal contents should be retained to avoid extrusion, otherwise the integrity of tissue structure will be affected.

3. The fixed tissue is directly dehydrated with absolute ethanol for a short time. Water-bearing ethanol should not be used for dehydration, otherwise the sugary mucous material will be lost.

4. Please tighten the cap in time after use to prevent volatile active ingredients.

5. For your safety and health, please wear a lab coat and disposable gloves when operating.

Jejunum in mice,AB-PAS,100X.

 Mice rectum,AB-PAS,100X.

 Jejunum in mice,PAS,100X