Electron Microscope Fixative

In histopathology, tissue must be fixed in time and effectively before subsequent embedding and sectioning. The role of tissue fixation is to maintain the inherent morphology and structure of tissues and cells, prevent bacterial corrosion and the autolysis of tissue cells, preserve the inherent substances in cells, solidify the proteins in cells, minimize or terminate the reaction of endogenous and exogenous enzymes, to keep the cells or tissues essentially as they were at the time of life, and can also harden the tissues and facilitate the repair of tissues. For some infectious specimens, fixation can prevent the spread of disease; preserve the gross specimen. Finally, fixation also enhance staining. Glutaraldehyde is a commonly used fixative, which preserves the fine structure of cells well, but its permeability is weak, which is mainly used for the early fixation of electron microscope samples.

This product is an electron microscope fixative with an active ingredient of 2.5% glutaraldehyde, pH 7.0-7.5 @ 25°C.

Ship with wet ice; Store at 2-8°C away from light, valid for 24 months.

Fresh tissues are quickly cut into 1-2 mm³ pieces, immerse in a sufficient amount of the fixative, fix at room temperature for 2h away from light, then transfer to 4°C. If the tissue is heavily laden with blood and tissue fluid, the tissue should be washed several times with the fixative, and then quickly cut into small pieces for fixation. If it is not possible to cut the tissue into 1-2 mm 3 pieces at once, it can also be cut into slightly larger tissue blocks, and then trimmed into 1-2 mm³size while fixing.

1. The tissue should be taken quickly to ensure that the tissue is taken from the living body and placed in a pre-cooled electron microscope fixative within 1 min. The operation is best carried out at low temperature ( 0-4°C ) to reduce enzyme activity and prevent cell autolysis.

2. Keep the tissue volume as small as possible, not more than 2 mm³, to ensure adequate penetration of the fixative into the tissue cells to obtain good fixation. Try to trim the tissue block in the fixative.

3. Please use sharp dissecting instruments to avoid ultrastructural damage caused by tissue pulling, contusion and compression.

4. Please use in a well-ventilated environment and take good protection to avoid inhalation.

5. Please tighten the cap in time after use to prevent volatilization of active ingredients

6. For your safety and health, please wear a lab coat and disposable gloves.