Special Fixative Solution For Fat

In histopathology, the tissue must be promptly and effectively fixed before subsequent embedding and sectioning can be completed. Organization fixed role is to maintain the inherent morphology and structure of organization, cell, prevent bacteria corrosion and tissue autolysis, save the inherent material inside the cell, the cell protein coagulation, as far as possible to reduce or end of endogenous and exogenous enzyme reaction, keep the cell or tissue basically like living material, also can make the organization hardening, easy to repair the cut of the organization. For certain infectious specimens, can prevent the spread of disease; Preserve the gross specimen. Finally, fixation can enhance the effect of staining. Formaldehyde is the most commonly used fixative, with strong penetration, uniform fixation, tissue shrinkage and appropriate hardness, which is suitable for the fixation of most tissue samples.

The traditional fixation method of testicular tissue is Bouin's fixation solution, but this fixation solution is acidic and has a great influence on antigen and nuclear staining, which is significantly affected. The testicular tissue fixed with paraformaldehyde solution was prone to interstitial loss. This fixative is specially used for the fixation of animal testis tissue with fast penetration, which can keep the structure of the tissue intact, retain most of the antigen, and do not affect the staining of the nucleus, and do not affect the detection of IHC or IF. The main components of this product are formaldehyde solution, ethanol and glacial acetic acid.

Transport at room temperature; Store in a cool and dry place, away from direct sunlight, valid for 24 months.

The fresh adipose tissue was removed and fixed in special adipose fixation solution for more than 24 h. The volume of fixed fluid was determined to be completely immersed in the tissue and the tissue could move freely. Due to the small density of adipose tissue, appropriate amount of absorbent cotton balls can be inserted on the surface of the fixation container to make the adipose tissue sink into the fixation fluid and get full fixation.

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