In Situ Hybridization Fixative Solution

In histopathology, tissue must be fixed in time and effectively before subsequent embedding and sectioning. The function of tissue fixation is to maintain the inherent morphology and structure of tissues and cells, prevent bacterial corrosion and autolysis of tissues and cells, preserve intrinsic substances in cells, coagulation of proteins in cells, minimize or terminate the reaction of endogenous and exogenous enzymes, so that cells or tissues basically maintain the same substances as life, but also make tissues harden, which is convenient for repair and cutting of thetissue. For some infectious specimens, fixative can prevent the spread of disease ; preserve the gross specimen. Finally, fixation can enhance the effect of staining.

The main components of the fixative solution for in situ hybridization (relatively tender plants) are formalin, glacial acetic acid and 50% ethanol, which are good for fixing plant tissues. It is suitable for more tender root, stem, leaf, anther and ovary tissue sections. DEPC treatment effectively inactivates RNase in the solution. It is specially used for fixing samples that need to be detected by nucleic acid in situ hybridization.

Normal temperature preservation and transportation ; valid for 24 months

The plant tissue was cut to a thickness of about 2 mm, and the cut tissue was placed into the in situ hybridization fixative (plant) at room temperature with tweezers or pipettes. The ratio of fixative to tissue was greater than 10 : 1. Fixed at room temperature for 12-24 h. If you can not cut into the size of 2 mm³at one time,can also be cut into slightly larger tissue blocks, and then fixed edge repair into the size of 2 mm³. Fixation time for smaller or tender tissues can be reduced appropriately according to the size of the material.

1. Please use in a well-ventilated environment, and take good protection to avoid inhalation.

2. In order to ensure that thefixative fully penetrates the tissue, vacuuming treatment can be added.

3. After use, please tighten the cap in time to prevent the effective ingredients from volatilize.

4. For your safety and health, please wear a lab coat and wear disposable gloves to operate.

                                                                                                                                                                                                                                  For Research Use Only!