MagBind Universal DNA Purification And Gel Extraction Kit

 

Product Name	Cat. No.	Spec.
MagBind Universal DNA Purification and Gel Extraction Kit	G3601-100T	100 T

 

 

MagBind Universal DNA Purification and Gel Extraction Kit utilizes the reversible binding of nucleic acids by magnetic beads and a unique buffer system for rapid and efficient purification of PCR products and digests, as well as isolation of specific DNA fragments of high purity from agarose gels. The entire operation does not require centrifugation, and more than 80% of the 100 bp-10 kb DNA fragments can be recovered in only 30 min. The purified DNA fragments can be applied to molecular biology experiments such as enzyme digestion reaction, ligation reaction, PCR amplification and DNA sequencing.

 

 

Ship at room temperature; store at room temperature for up to 1 year.

 

 

Component Number	Component	G3601-100T
G3601-1	Buffer SPS	50 mL
G3601-2	SweMag Beads	6 mL
G3601-3	Buffer SPW	24 mL (56 mL of anhydrous ethanol added before use)
G3601-4	Buffer TE	10 mL
Manual		One copy

 

 

1. Add the specified amount (see bottle) of anhydrous ethanol to Buffer SPW before use.

2. Magnetic stands and sterilized 1.5 mL centrifuge tubes are required but not supplied in this kit.

 

 

Recovery of DNA fragments from agarose gels

1. Cut the gel block containing the specific DNA bands from the agarose gel, removing as much excess as possible (do not expose the gel block to UV light for a long time when cutting to prevent DNA damage).

2. Add an amount of Buffer SPS to the centrifuge tube (e.g., 100 µL of Buffer SPS if the weight of the cut gel block is 0.1 g) and heat at 60°C for 5-10 min, during which time the tube is continually turned up and down until the gel block is completely dissolved.

3. Add equal volume of absolute ethanol to the gel mixture, mix well with pipette or votex mixer, and then add 0.6 times the volume of PCR or enzyme digestion volume of SweMag Beads (SweMag Beads need to be vortexed until well dispersed prior to use) and mix well using a pipette or vortexer;

4. Stand at room temperature for 5 minutes and mix well with pipette or votex mixer every 2-3 times to help SweMag Beads evenly distributed.

5. Place the tube on a magnetic stand for 30 seconds until the magnetic beads are completely attached. Gently invert the centrifuge tube with the magnetic stand up and down several times to wash away residual magnetic beads. When the supernatant is clear, aspirate and discard the clear supernatant (To avoid lower recovery rate, do not aspirate the magnetic beads).

6. Add 300 μL Buffer SPW, remove the magnetic stand, and gently blow with a pipette until the magnetic beads are well dispersed. Place the tube on a magnetic stand for 30 seconds and gently invert the centrifuge tube with the magnetic stand up and down several times to wash away residual magnetic beads and salts on the tube wall, and then aspirate the supernatant when it is clear (to avoid affecting the efficiency of the extraction, be sure to aspirate the residual liquid in the centrifuge tube).

7. Repeat step 6.

8. Open the centrifuge tube lid, place it at 65℃ for 3-5 minutes or leave it at room temperature for 5-10 minutes to ensure complete evaporation of residual ethanol (Do not allow them to dry out to avoid affecting the nucleic acid yield).

9. Remove the magnetic stand, add 30–50 μL Buffer TE or Nuclease-free Water and pipet up and down gently to resuspend the magnetic beads, stand at room temperature for 3 minutes.

10. Place the tube on a magnetic stand until the magnetic beads are fully adsorbed, then aspirate the supernatant to a new centrifuge tube to obtain high purity DNA.

DNA extraction from PCR or restriction enzyme digestion product

1. Add 2 volume of Buffer SPS and absolute ethanol respectively to 1 volume of PCR or restriction enzyme digestion product, mix well with pipette or votex mixer. Add 0.6 volume SweMag Beads suspension to per 1 volume of PCR or restriction enzyme digestion product and mix well (SweMag Beads must be fully resuspended and evenly distributed prior to use), mix well with pipette or votex mixer.

2. Stand at room temperature for 5 minutes and mix well with pipette or votex mixer every 2-3 times to help SweMag Beads evenly distributed.

3. Place the tube on a magnetic stand for 30 seconds until the magnetic beads are completely attached. Gently invert the centrifuge tube with the magnetic stand up and down several times to wash away residual magnetic beads on the tube wall. Aspirate and discard the clear supernatant when it is clear (To avoid lower recovery rate, do not aspirate the magnetic beads).

4. Add 300 μL Buffer SPW, remove the magnetic stand, pipet up and down gently to resuspend the magnetic beads. Place the tube on a magnetic stand for 30 seconds and gently invert the centrifuge tube with the magnetic stand up and down several times to wash away residual magnetic beads and salts on the tube wall. Aspirate and discard the clear supernatant when it is clear (to avoid affecting the efficiency of the extraction, be sure to aspirate the residual liquid in the centrifuge tube).

5. Repeat step 4.

6. Open the centrifuge tube lid, place it at 65℃ for 3-5 minutes or leave it at room temperature for 5-10 minutes to ensure complete evaporation of residual ethanol (Do not allow them to dry out to avoid affecting the nucleic acid yield).

7. Remove the magnetic stand, add 30–50 μL Buffer TE or Nuclease-free Water and pipet up and down gently to resuspend the magnetic beads, stand at room temperature for 3 minutes.

8. Place the tube on a magnetic stand until the magnetic beads are fully adsorbed, then aspirate the supernatant to a new centrifuge tube to obtain high purity DNA.

 

 

1. Please read the Product Manual carefully before use.

2. Magnetic bead suspensions should be stored to avoid freezing.

3. The beads are prone to precipitation and should be shaken or vortexed to mix well before use.

4. Before adding the magnetic beads, other reagents need to be mixed well.

5. Ethanol should be completely evaporate before DNA elution due to residual ethanol affecting downstream experiments.

6. Do not dry the beads for a long time as this may affect the DNA elution efficiency.

7. For your safty and health, please wear safety glasses, gloves, or protective clothing.

 

 

Volume of PCR or restriction enzyme digestion product	SweMag Beads	Number of reactions
50 µL	30 µL	200
100 µL	60 µL	100

 

For Research Use Only!