SerBlue Nucleic Acid Dye (10000×, Water Soluble)

SerBlue has a unique molecular structure and is a highly sensitive, non-mutagenic, ultra-safe and ultra-stable fluorescent nucleic acid gel staining reagent for the detection of ssDNA, RNA and dsDNA in agarose gel, which can replace unsafe nucleic acid dyes such as ethidium bromide (EtBr, EB) with much higher sensitivity than EB and lower background signal. There is no need for decolorization, even if the amount of DNA samples is very high, the band separation effect can be obtained. The dye can be excited by a 488 nm laser and directly observed with a blue light Gel cutter or a blue light scanner.

Ship and store at room temperature; valid for 24 months.

1. Gel dyeing method

a. To prepare the gel, add 5 μL of SerBlue Nucleic Acid Dye (10,000 x, Water Soluble) per 50 mL of agarose (GC205013 recommended) gel (SerBlue has excellent thermal stability and can be added directly to the high-temperature gel solution without waiting for the gel solution to cool. It can also be made by pre-mixing SerBlue with an electrophoresis buffer containing agarose powder and heating it), and mix thoroughly, then poured, and wait for agarose gel to completely solidify for sample electrophoresis.

b. Electrophoresis according to conventional methods, followed by blue light imaging.

2. Soak dyeing method

a. Electrophoresis according to conventional methods.

b. Dilute the SerBlue Nucleic Acid Dye (10000×, Water Soluble) stock solution approximately 3300 times into 0.1 M NaCl aqueous solution to make a 3× staining solution (for example, add 15 μL of SerBlue Nucleic Acid Dye 10000× stock solution to 50 mL of 0.1 M NaCl aqueous solution).

c. Carefully place the gel into a suitable container and soak the gel with a sufficient amount of 3 x staining solution, and incubate it for 30min in a shaker at room temperature away from light. If it is acrylamide gel, incubated for 30min to 1h, and extended with the increase of acrylamide content.

d. After staining, blue light imaging.

1. Immediately centrifuge the product to the bottom of the tube before use, and then conduct subsequent experiments.

2. The dye does not need to be refrigerated at low temperature. Please store it at room temperature to avoid precipitation. If precipitation is found, please heat the dyes to 45-50°C for 2 min and shake to dissolve, which does not affect the use.

3. The gel prepared by the gel dyeing method is light orange red. After electrophoresis, the gel color may be uneven when observed by the naked eye (the upper part of the gel color is dark, the lower part of the gel color is light), which is a normal phenomenon and does not affect the electrophoresis result.

4. SerBlue can be used not only for agarose gel electrophoresis, but also for acrylamide gel nucleic acid electrophoresis.

5. SerBlue is suitable for blue light Gel cutting machines and blue light scanners, and can also be used in ultraviolet gel imaging systems, but the ultraviolet imaging bands are darker.

6. This product can stain single-stranded DNA and RNA, but is less sensitive to single-stranded DNA or RNA than double-stranded DNA.

7. The dyeing solution can be reused for about 3 times, and it is recommended to store the used dyeing solution that needs to be used continuously away from light.

8. It is recommended that operators use it in specific areas, take protective measures, wear gloves and masks, and properly dispose of waste.

For Research Use Only!