Immunoprecipitation Kit (Protein G Agarose)

IP or Co-IP is a common experimental technique for studying protein or protein-protein interactions (PPIs) by using specific antibodies and a medium that binds antibodies (e.g. Protein A/G Agarose or Protein A/G Magrose), or directly using a medium coupled with specific antibodies (e.g. agarose or magnetic beads), and then isolating the antigen-antibody complexes from the complicated protein samples by centrifugation or magnetic force, so as to achieve the purpose of isolation and purification of the target proteins, which can subsequently be used for Western blot or mass spectrometry. Protein A is a cell wall surface protein found in Staphylococcus aureus with a molecular weight of 42 kDa. Protein G is an immunoglobulin binding protein expressed by Streptococcal bacteria type C or G. Protein A and Protein G are functionally similar and can bind specifically to mammalian immunoglobulin (Ig). Recombinant Protein A and G with appropriate modifications bound to agarose can be used for immunoprecipitation or antibody purification. Protein A agarose are suitable for the immunoprecipitation of human IgG1, IgG2, IgG4, mouse IgG2a, IgG2b, while Protein G agarose beads are suitable for the immunoprecipitation of human IgG1, IgG2, IgG3, IgG4, mouse IgG1, IgG2a, IgG2b, IgG3, rat IgG1, IgG2a, IgG2b, IgG2c polyclonal antibodies. (See Table I for specific information)

This product adopts the self-developed and produced Protein G labelled agarose, compared with similar products in the market, this product binds antibodies more efficiently and has a lower non-specific binding rate, together with the optimized buffer, it can be convenient and efficient for immunoprecipitation experiments; it can be widely used in the isolation and purification of the target proteins in samples such as cell lysates, cellular secretion supernatants, serum, ascites, etc.; This kit provides two elution methods (denaturing elution and acid elution) to elute the target protein, especially the acid elution will not contain the light and heavy chains of the antibody, which can effectively solve the problem of the interference of the antibody's heavy and light chains in the immunoprecipitation and protein immunoblotting experiments.

Table 1

For research use only. Not for use in diagnostic or therapeutic procedures!