GoldView Nucleic Acid Dye (10000×, Water Soluble)

This product is a cellular permeable nucleic acid binding dye that can replace ethidium bromide (EtBr, EB). When performing agarose gel electrophoresis, it emits strong green fluorescence after binding with double-stranded DNA and red fluorescence after binding with single-stranded DNA or RNA. The sensitivity is equivalent to EB and the method of use is exactly the same. Goldview is particularly suitable for the detection of large fragments (> 1kb) of DNA, with reduced sensitivity at less than 500bp.

Ship and store at room temperature; valid for 24 months.

1. Gel dyeing method

a. To prepare the gel, add 5-10 μL of GoldView Nucleic Acid Dye (10,000 x, Water Soluble) per 50 mL of agarose (GC205013 recommended) gel when cooling to not hot,and mix thoroughly, then poured, and wait for agarose gel to completely solidify for sample electrophoresis.

b. After electrophoresis, observations were made using a gel imaging system or visible light transmissometer.

2. Soak dyeing method

a. Electrophoresis according to conventional methods.

b. Dilute the GoldView Nucleic Acid Dye (10000×, Water Soluble) to 1× (for example, add 5 μL of GoldView Nucleic Acid Dye 10000× stock solution to 50 mL of 1× electrophoresis buffer).

c. Carefully place the gel into a suitable container and soak the gel with a sufficient amount of 1 x staining solution, and incubate it for 10min to 30min in a shaker at room temperature away from light. (the specific time depends on the thickness of the gel and the amount of nucleic acid) If it is acrylamide gel, incubated for 30min to 1h, and extended with the increase of acrylamide content..

d. After dyeing, if necessary, the gel can be shaken in water for 30 minutes to remove the color.

e. Observations are made using gel imaging systems or visible light transmissometers.

1. The dye does not need to be refrigerated at low temperature. Please store it at room temperature away from light.

2. Immediately centrifuge the product to the bottom of the tube before use, and then conduct subsequent experiments.

3. The main component of Goldview is acridine orange, which has cell permeability and has certain cytotoxicity.

4. Gels containing Goldview nucleic acid dyes are not suitable for gel recovery experiments.

5. Gel dyeing method reduces the mobility of nucleic acids.

6. Gel thickness should not exceed 0.5 cm, too thick Gel will affect the sensitivity of detection.

7. Don't repeatedly melt agaroses that already contain Goldview, which will reduce the sensitivity of nucleic acid detection.

8. The dyeing solution can be reused for about 3 times, and it is recommended to store the used dyeing solution that needs to be used continuously away from light.

9. It is recommended that operators use it in specific areas, take protective measures, wear gloves and masks, and properly dispose of waste.

For Research Use Only!