Product Introduction
|
Product Name |
Cat. No. |
Spec. |
|
10×MOPS Buffer |
G3373-100ML |
100 mL |
Product Description/Introduction
10×MOPS Buffer is mainly composed of MOPS, sodium acetate, EDTA, formulated with nuclease-free water and filtered by microporous membrane, suitable for RNA formaldehyde denaturation electrophoresis. This product is tested to have no RNase activity.
This product contains 0.4 M MOPS, 0.1 M sodium acetate, 10 mM EDTA, pH 7.0 ± 0.1.
Storage and Shipping Conditions
Ship and store at room temperature; Avoid direct sunlight, valid for 24 months.
Assay Protocol / Procedures
This product is a ten-fold concentrate and needs to be used after 10-fold dilution, i.e., every 10 mL of 10×MOPS Buffer is mixed with 90 mL of nuclease-free water (recommended G4700 or G3004).
When used to prepare agarose gel for RNA formaldehyde denaturation electrophoresis, it can be operated as follows: Take 1% agarose gel preparation as an example, weigh 0.5 g of agarose and add it to 36 mL of RNase-Free Water (recommended G4700 or G3004), heat and dissolve it, and then add 5 mL of 10×MOPS Buffer (recommended G3373). When the solution is cooled until it is not hot, add 9 mL of 37% formaldehyde solution, add nucleic acid dye EB as needed, mix well, and pour the gel.
Note
1. Handle with care and use sterile, enzyme-free pipette tips to aspirate the solution to avoid RNase contamination.
2. The product is easy to turn yellow when exposed to light, so it is recommended to store in a cool and dark place. When the solution is light yellow, it does not affect to use. If the color is too dark, it is recommended to stop using.
3. For your safety and health, please wear protective clothing and gloves.
For Research Use Only!
