PVDF Membrane (0.2μm)

PVDF membrane is polyvinylidene fluoride membrane (polyvinylidene fluoride), which has high mechanical strength and is an ideal solid support in Western blotting. PVDF membrane is hydrophobic, and the pore size of the membrane is large or small. The smaller the pore size of the membrane, the stronger the binding of proteins with low molecular weight. According to the pore size of the membrane, there are generally different specifications such as 0.1 μm, 0.22 μm, 0.45 μm and 1.0 μm. The 0.22 μm PVDF membrane is mainly used for low molecular weight Western blotting, amino acid analysis and N-terminal protein sequencing.

This product is 0.22 μm aperture, imported original, 26.5 cm×3.75 m/roll. Suitable for most western blots, especially for proteins with molecular weights less than 20 kDa.

Transport at room temperature, keep dry and sealed to prevent moisture. long term effective.

1. A membrane with a pore size of 0.45 μm is recommended for proteins with a molecular weight greater than 20 kDa, and a 0.22 μm membrane for proteins with a molecular weight of less than 20 kDa.

2. The PVDF membrane needs to be pretreated with methanol before use to activate the positively charged groups on the membrane, making it easier to bind to negatively charged proteins.

1. The PVDF membrane is easy to dry and should be placed in TBST as soon as possible after transfer. If the membrane is too dry, it is recommended to reactivate it with methanol.

2. Please wear a lab coat and disposable gloves during operation.