Prodoct Information
Product Information
|
Protein full name |
T-cell surface glycoprotein CD3 epsilon chain |
|
Synonyms |
CD3E, IMD18, T3E, TCRE, CD3e molecule |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse CD3 |
|
Uniprot ID |
P22646 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Cell membrane |
|
Predicted MW. / Observed MW. |
21 kDa / 21 kDa |
Applications
|
Applications |
Species |
Dilution |
Positive Sample |
|
WB |
Human, Mouse, Rat |
1:500-1:1000 |
thymus, lymph node |
|
IHC/IF |
Human, Mouse, Rat |
1:250-1:1000 |
spleen, splenitis, tonsil |
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.
Images
|
|
Western blot analysis of CD3 (GB11014) at dilution of 1: 500 |
 |
Immunohistochemistry of paraffin embedded human tonsil using CD3 (GB11014) at dilution of 1: 1000 (400x lens) |
 |
Immunohistochemistry of paraffin embedded mouse spleen using CD3 (GB11014) at dilution of 1: 1000 (400x lens) |
 |
Immunohistochemistry of paraffin embedded mouse splenitis using CD3 (GB11014) at dilution of 1: 1000 (400x lens) |
 |
Immunohistochemistry of paraffin embedded rat spleen using CD3 (GB11014) at dilution of 1: 1000 (400x lens) |
 |
Immunofluorescence of paraffin embedded human tonsil using CD3 (GB11014) at dilution of 1: 500 (200x lens) |
 |
Immunofluorescence of paraffin embedded human tonsil using CD3 (GB11014) at dilution of 1: 500 (400x lens) |
 |
Immunofluorescence of paraffin embedded mouse spleen using CD3 (GB11014) at dilution of 1: 500 (400x lens) |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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