Prodoct Information
|
Protein full name |
Caspase-9 |
|
Synonyms |
CASP9, APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56, caspase 9 |
|
Immunogen |
Recombinant protein corresponding to Mouse Caspase-9 |
|
Uniprot ID |
Q8C3Q9 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm, Nucleus |
|
Predicted MW. / Observed MW. |
50 kDa(M) / 50 kDa(M) |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Mouse, Rat |
1: 800-1: 1000 |
heart, brain, skeletal muscle |
Background
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts (inactive form) with EFHD2. Interacts with HAX1. Interacts with BIRC2/c-IAP1, XIAP/BIRC4, BIRC5/survivin, BIRC6/bruce and BIRC7/livin. Interacts with ABL1 (via SH3 domain); the interaction is direct and increased in the response of cells to genotoxic stress and ABL1/c-Abl activation.
Images
|
|
Western blot analysis of Caspase9 (GB11053-1) at dilution of 1: 800 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
