Anti-F4/80 Rabbit PAb

Product Introduction:

Cat.No.：GB11027-100

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Description

Technical Parameters

Prodoct Information

Description	F4/80 rabbit polyclonal antibody
Protein full name	Adhesion G protein-coupled receptor E1
Synonyms	ADGRE1, TM7LN3, EMR1, Gpf480, adhesion G protein-coupled receptor E1
Immunogen	Recombinant protein corresponding to Mouse F4/80
Isotype	IgG
Purity	Affinity purification
Subcellular location	Cell membrane
Uniprot ID	Q61549

Applications

Applications	Species	Dilution	Positive Sample
IHC/IF	Mouse	1: 500-1: 2000	liver, lung

Background

EGF-like module-containing mucin-like hormone receptor-like 1 also known as F4/80 is a proteinencoded by the ADGRE1 gene. EMR1 is a member of the adhesion GPCR family.Adhesion GPCRs are characterized by an extended extracellular region often possessing N-terminal protein modules that is linked to a TM7 region via a domain known as the GPCR-Autoproteolysis INducing (GAIN) domain.

Images

	IHC analysis of F480 (GB11027). Sample: Mouse liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1200, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
	IHC analysis of F480 (GB11027). Sample: Mouse lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.
	IF analysis of F4-80 (GB11027). Sample: Mouse liver+lps (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98℃,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h.

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).