BTT Fast-Cast Colorful (Red) Acrylamide Kit,10%

 

Product Name	Cat. No.	Spec.
BTT Fast-Cast Colorful (Red) Acrylamide Kit, 10%	G2067-50T	50 T

 

 

This kit provides a simple and rapid preparation of 10% PAGE coloured (red) gels, enabling the simultaneous preparation of both resolving and stacking gels, allowing the user to prepare protein gels quickly by simply preparing the gel preparation equipment. At the same time, the kit contains a red dye in the stacker B, which makes it possible to visualise the spiking process and greatly improves the spiking efficiency. The gels can be electrophoresed at a constant pressure of 200 V and take only 30 min to complete the electrophoresis process, which is less time consuming and provides better resolution than conventional gel electrophoresis systems. The gels prepared in this kit can also be used for non-denaturing PAGE gel electrophoresis.

This method does not need to add TEMED by itself when preparing, only the modified coagulant can form the gel, which greatly reduces the harm of TEMED to human body and the environment. This product is a modified Bis-Tris formulation gel, which needs to be used with matching electrophoresis buffer, and Tris-Glycine electrophoresis buffer cannot be used. The specific number of prepared gel blocks is related to the thickness of the gel and the size of the gel.

 

 

Ship with wet ice; Modified procoagulant need to be stored at -20 ℃, 25×Tris-MOPS SDS-PAGE buffer can be stored at room temperature; Other components need to be stored at 2-8 ℃ to avoid light, validity for 12 months.

 

 

Component Number	Component	G2067
G2067-1	BTT Stacker A	50 mL
G2067-2	BTT Stacker B(Red)	50 mL
G2067-3	10% BTT Resolver A	125 mL
G2067-4	10% BTT Resolver A	125 mL
G5036-5ML	Modified Coagulant	1 mL×5
G2050-250ML	25×Tris-MOPS SDS-PAGE Buffer Solution	500 mL
User manual		1 pc

 

 

1. Choose appropriate Concentration of PAGE resolver (separation gel) according to molecular weight of target protein, referring to the table below:

Concentration of acrylamide	Separation range
8%	≥35 kDa
10%	10-250 kDa
12%	10-100 kDa

 

2. According to the experimental requirements, mix solution A and B in equal proportion, add appropriate amount of modified coagulant, and prepare the resolving solution and stacking solution respectively. Different specifications and different thickness of the glass plate can be adjusted in equal proportions to the stacking solution and resolving solution preparation volume, to the commonly used specifications of 8.3 cm × 7.3 cm gel plate (single), for example, the recommended preparation system is as follows table;

Configuration Groups	Components	0.75 mm Glass Plates	1.0 mm Glass Plates	1.5 mm Glass Plates
Resolver	BTT 10% Resolver A	2 mL	2.5 mL	4 mL
	BTT 10% Resolver B	2 mL	2.5 mL	4 mL
	Modified Coagulant	24 μL	30 μL	48 μL
Stacker	BTT Stacker A	1 mL	1 mL	1.5 mL
	BTT Stacker B (Red)	1 mL	1 mL	1.5 mL
	Modified Coagulant	12 μL	12 μL	18 μL

 

3. After assembling the gel tool, first add the prepared resolver, then use distilled water or ethanol and other solutions to seal the surface of the resolver, wait until the resolver to solidify sufficiently (about 10-15 min), discard the water or ethanol, and use the filter paper to suck up the residual water or ethanol, and then add the prepared stacker, insert the comb, and wait until it is solidified (about 10-15 min), and then can be used; or in the stacker can be added to join the prepared resolver immediately and slowly, uniformly added to the upper layer of the gel solution prepared, and insert the comb, and wait until it is solidified (about 10-15 min), and then can be used;

4. If the prepared gel cannot be used on the same day, it can be stored at 4℃ for 1-2 days;

5. Dilute BTT electrophoresis buffer (25×) into 1× electrophoresis buffer before electrophoresis (it is not recommended to reuse the electrophoresis buffer more than 2 times);

6. The electrophoresis can be done at constant voltage of 200 V throughout the electrophoresis, and the electrophoresis can be completed in about 30 minutes in general.

 

 

1. Modified coagulant is more stable than ammonium persulfate (AP), take out one when using, save it at 4℃ after using for subsequent routine use, it can be saved for six months; if it is not used for a long time, please save it at -20℃ to avoid repeated freezing and thawing;

2. There is monomer acrylamide in the premixed solution, which is harmful to human body, please pay attention to the protective measures during operation;

3. In order to ensure that the samples enter the separation gel at the same time, when directly filling the stacker, it is necessary to slowly and evenly add the prepared stacker;

4. Fill the gel as soon as possible after adding the modified coagulant promoter, do not leave it for a long time;

5. If you need to further accelerate the gelation speed, you can increase the amount of modified coagulant by 0.5 times.

6. The temperature has a big influence on the solidification time of the gel, in order to ensure the smooth running of the experiment, generally the lower the temperature, the longer the solidification time, you can appropriately increase the dosage of modified coagulant; the higher the temperature, the faster the gel, you can appropriately reduce the dosage of modified coagulant;

7. Our company also provides other colored (red, yellow, blue) sracker to distinguish different samples of different gel electrophoresis;

8. The gel made by this kit is not compatible with Tris-Glycine electrophoresis buffer system, and can only be used with Tris-MOPS SDS-PAGE electrophoresis buffer included in the kit, and a single product of Tris-MOPS SDS-PAGE electrophoresis buffer (25×) is provided separately, for details, see G2050.

 

For Research Use Only!